Applied Microbiology and Biotechnology

, Volume 97, Issue 24, pp 10555–10561 | Cite as

UV photolysis for accelerated quinoline biodegradation and mineralization

  • Ning Yan
  • Ling Chang
  • Lu Gan
  • Yongming Zhang
  • Rui Liu
  • Bruce E. Rittmann
Environmental biotechnology


Sequentially and intimately coupled photolysis with biodegradation were evaluated for their ability to accelerate quinoline-removal and quinoline-mineralization kinetics. UV photolysis sequentially coupled to biodegradation significantly improved biomass-growth kinetics, which could be represented well by the Aiba self-inhibition model: UV photolysis increased the maximum specific growth rate (μ max) by 15 %, and the inhibition constant (K SI) doubled. An internal loop photo-biodegradation reactor (ILPBR) was used to realize intimately coupled photolysis with biodegradation. The ILPBR was operated with batch experiments following three protocols: photolysis alone (P), biodegradation alone (B), and intimately coupled photolysis and biodegradation (P&B). For P&B, the maximum quinoline removal rate (r max) increased by 9 %, K SI increased by 17 %, and the half-maximum-rate concentration (K S) decreased by 55 %, compared to B; the composite result was a doubling of the quinoline-biodegradation rate for most of the concentration range tested. The degree of mineralization was increased by both forms of photolysis coupled to biodegradation, and the impact was greater for intimate coupling (18 % increase) than sequential coupling (5 %). The benefits of UV photolysis were greater with intimate coupling than with sequential coupling due to parallel transformation by biodegradation and photolysis.


Quinoline Biodegradation Photolysis Mineralization Kinetics 



The authors acknowledge the financial support by the National Natural Science Foundation of China (50978164), Key project of basic research in Shanghai (11JC1409100), the Special Foundation of Chinese Colleges and Universities Doctoral Discipline (20113127110002), Innovation Fund for Key Projects of Shanghai Municipal Education Commission (10ZZ82), Program of Shanghai Normal University (DZL123), and the United States National Science Foundation (0651794).


  1. Aiba S, Shoda M, Nagalani M (2000) Kinetics of product inhibition in alcohol fermentation. Biotechnol Bioeng 67(6):671–690PubMedCrossRefGoogle Scholar
  2. Alinsafi A, Evenou F, Abdulkarim EM, Pons MN, Zahraa O, Benhammou A, Yaacoubi A, Nejmeddine A (2007) Treatment of textile industry wastewater by supported photocatalysis. Dyes Pigments 74(2):439–445CrossRefGoogle Scholar
  3. American Public Health Association (APHA) (2001) Standard Methods for the Examination of Water and Wastewater, 22nd Edition USA. American Water Works Association and Water Pollution Control Federation, Washington, DCGoogle Scholar
  4. An T, Zhang W, Xiao X, Sheng G, Fu J, Zhu X (2004) Photoelectrocatalytic degradation of quinoline with a novel three-dimensional electrode-packed bed photocatalytic reactor. J Photoch Photobio A 161(2–3):233–242CrossRefGoogle Scholar
  5. Aranda C, Godoy F, Becerra J, Barra R, Martínez M (2003) Aerobic secondary utilization of a non-growth and inhibitory substrate 2,4,6-trichlorophenol by Sphingopyxis chilensis S37 and sphingopyxis-like strain S32. Biodegradation 14(4):265–274PubMedCrossRefGoogle Scholar
  6. Bai Y, Sun Q, Zhao C, Wen D, Tang X (2010) Quinoline biodegradation and its nitrogen transformation pathway by a Pseudomonas sp. strain. Biodegradation 21(3):335–344PubMedCrossRefGoogle Scholar
  7. Bleeker EAJ, van der Geest HG, Kraak MHS, de Voogt P, Admiraal W (1998) Comparative ecotoxicity of NPAHs to larvae of the midge Chironomus riparius. Aquat Toxicol 41:51–62CrossRefGoogle Scholar
  8. Bohlmann U, Bohnet M (2001) Improvement of process stability of microbiological quinoline degradation in a three-phase fluidized bed reactor. Eng Life Sci 1(2):91–96CrossRefGoogle Scholar
  9. Buchtmann C, Kies U, Deckwer WD, Hecht V (1997) Performance of three phase fluidized bed reactor for quinoline degradation on various supports at steady state and dynamic conditions. Biotechnol Bioeng 56(3):295–303PubMedCrossRefGoogle Scholar
  10. Cao B, Nagarajan K, Loh KC (2009) Biodegradation of aromatic compounds: current status and opportunities for biomolecular approaches. Appl Microbiol Biot 85(2):207–228CrossRefGoogle Scholar
  11. Fetzner S (1998) Bacterial degradation of pyridine, indole, quinoline, and their derivatives under different redox conditions. Appl Microbiol Biot 49(3):237–250CrossRefGoogle Scholar
  12. Kaiser JP, Feng YC, Bollag JM (1996) Microbial metabolism of pyridine, quinoline, acridine, and their derivatives under aerobic and anaerobic conditions. Microbiol Rev 60(3):483–498PubMedGoogle Scholar
  13. Kim TS, Kim JK, Choi K, Stenstrom MK, Zoh KD (2006) Degradation mechanism and the toxicity assessment in TiO2 photocatalysis and photolysis of parathion. Chemosphere 62(6):926–933PubMedCrossRefGoogle Scholar
  14. Marsolek MD, Torres CI, Hausner M, Rittmann EB (2008) Intimate coupling of photocatalysis and biodegradation in a photocatalytic circulating-bed biofilm reactor. Biotechnol Bioeng 101(1):83–92PubMedCrossRefGoogle Scholar
  15. Miethling R, Hecht V, Deckwer WD (1993) Microbial degradation of quinoline: kinetic studies with Comamonas acidovorans DSM 6426. Biotechnol Bioeng 42(5):589–595PubMedCrossRefGoogle Scholar
  16. Minako N, Takio Y, Yuko S, Takashi S (1977) Mutagenicities of quinoline and its derivatives. Mutat Res 42:335–342CrossRefGoogle Scholar
  17. Mohanty S, Rao NN, Khare P, Kaul SN (2005) A coupled photocatalytic–biological process for degradation of 1-amino-8-naphthol-3, 6-disulfonic acid (H-acid). Wat Res 39(20):5064–5070CrossRefGoogle Scholar
  18. Namkung E, Rittmann BE (1987) Evalution of bisubstrate secondary utilization kinetics by biofilms. Biotech and Bioeng 29:335–342CrossRefGoogle Scholar
  19. Ogunsola OM (2000) Decomposition of isoquinoline and quinoline by supercritical water. J Hazard Mater 74(3):187–195PubMedCrossRefGoogle Scholar
  20. Qi Y, Wang S (2007) Biological reaction kinetics and reactor, 3rd edn. Chemical Industry Press, Beijing (in Chinese)Google Scholar
  21. Qiao L, Wang J (2010) Biodegradation characteristics of quinoline by Pseudomonas putida. Bioresour Technol 101(19):7683–7686CrossRefGoogle Scholar
  22. Remoundaki E, Vidali R, Kousi P (2009) Photolytic and photocatalytic alterations of humic substances in UV (254 nm) and Solar Cocentric Parabolic Concentrator (CPC) reactors. Desalination 248(1–3):843–851CrossRefGoogle Scholar
  23. Sideropoulos AS, Secht SM (1984) Evaluation of microbial testing methods for the mutagenicity of quinoline and its derivatives. Curr Microbiol 11(2):59–66CrossRefGoogle Scholar
  24. Sun Q, Bai Y, Zhao C, Xiao Y, Wen D, Tang X (2009) Aerobic biodegradation characteristics and metabolic products of quinoline by a Pseudomonas strain. Bioresour Technol 100(21):5030–5036PubMedCrossRefGoogle Scholar
  25. Sutton SD, Pfaller SL, Shann JR, Warshawsky D, Kinkle BK, Vestal JR (1996) Aerobic biodegradation of 4-methylquino-line by a soil bacterium. Appl Environ Microb 62(8):2910–2914Google Scholar
  26. Thomas JK, Gunda K, Rehbein P, Flora TTN (2010) Flow calorimetry and adsorption study of dibenzothiophene, quinoline and naphthalene over modified Y zeolites. Appl Catal B- Environ 94(3–4):225–233CrossRefGoogle Scholar
  27. Thomsen AB (1998) Degradation of quinoline by wet oxidation—kinetic aspects and reaction mechanisms. Wat Res 32(1):136–146CrossRefGoogle Scholar
  28. von Stosch M, Oliveria R, Peres J (2012) Hybrid modeling framework for process analytical technology: application to Bordetella pertussis cultures. Biotechnol Prog 28(1):284–291CrossRefGoogle Scholar
  29. Wan M, Wang R, Xia J (2012) Physiological evaluation of a new Chlorella sorokiniana isolate for its biomass production and lipid accumulation in photoautotrophic and heterotrophic cultures. Biotechnol Bioeng 109(8):1958–1964PubMedCrossRefGoogle Scholar
  30. Wang X, Huang X, Zuo C, Hu H (2004) Kinetics of quinoline degradation by O3/UV in aqueous phase. Chemosphere 55(5):733–741PubMedCrossRefGoogle Scholar
  31. Xing X, Zhu X, Li H, Jiang Y, Ni J (2012) Electrochemical oxidation of nitrogen-heterocyclic compounds at boron-doped diamond electrode. Chemosphere 86(4):368–375PubMedCrossRefGoogle Scholar
  32. Yan N, Xia S, Xu L, Zhu J, Zhang Y, Rittmann BE (2012) Internal loop photobiodegradation reactor (ILPBR) for accelerated degradation of sulfamethoxazole (SMX). Appl Microbiol Biotechnol 94(2):527–535PubMedCrossRefGoogle Scholar
  33. Yuan F, Hu C, Hu X, Wei D, Chen Y, Qu J (2011) Photodegradation and toxicity changes of antibiotics in UV and UV/H2O2 process. J Hazard Mater 185(2–3):1256–1263PubMedCrossRefGoogle Scholar
  34. Zhang Y, Pu X, Fang M, Zhu J, Chen L, Rittmann BE (2012) 2,4,6-Trichlorophenol (TCP) photo biodegradation and its effect on community structure. Biodegradation 23(4):575–583PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Ning Yan
    • 1
  • Ling Chang
    • 1
  • Lu Gan
    • 1
  • Yongming Zhang
    • 1
  • Rui Liu
    • 2
  • Bruce E. Rittmann
    • 3
  1. 1.Department of Environmental EngineeringCollege of Life and Environmental Science, Shanghai Normal UniversityShanghaiPeople’s Republic of China
  2. 2.Zhejiang Provincial Key Laboratory of Water Science and Technology, Department of Environmental Technology and EcologyYangtze Delta Region Institute of Tsinghua UniversityJiaxingChina
  3. 3.Swette Center for Environmental BiotechnologyBiodesign Institute, Arizona State UniversityTempeUSA

Personalised recommendations