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Applied Microbiology and Biotechnology

, Volume 97, Issue 21, pp 9429–9437 | Cite as

Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen

  • Outi Nivala
  • Maija-Liisa Mattinen
  • Greta Faccio
  • Johanna Buchert
  • Kristiina Kruus
Biotechnologically relevant enzymes and proteins

Abstract

Sulfhydryl oxidases (SOX) are FAD-dependent enzymes capable of oxidising free thiol groups and forming disulphide bonds. Although the quantity of scientific papers and suggested applications for SOX is constantly increasing, only a limited number of microbial SOX have been reported and are commercially available. Hence, the aim of this study was to develop a fast and reliable qualitative plate test for screening novel secreted fungal SOX. The screening was based on the Ellman's reagent, i.e. 5,5′-dithiobis[2-nitrobenzoic acid]. Altogether, 32 fungal strains from an in-house culture collection were screened. A total of 13 SOX-producing strains were found positive in the plate test screen. The novel SOX producers were Aspergillus tubingensis, Chaetomium globusum, Melanocarpus albomyces, Penicillium aurantiogriseum, Penicillium funiculosum, Coniophora puteana and Trametes hirsuta. Six of the discovered SOX were partially characterised by determination of isoelectric point, pH optimum and substrate specificity. A. tubingensis was identified as the most efficient novel SOX producer.

Keywords

Fungi Enzyme Sulfhydryl oxidase Oxidation Thiol Ellman's reagent Screening 

Notes

Acknowledgements

The research was supported by Academy of Finland: Enzymatic cross-linking of food proteins: impact of food protein folding on the mode of action of cross-linking enzymes (No. 110965), EU: High performance industrial protein matrices (No. NMP-3-CT-2003-505790) and TEKES (The Finnish Funding Agency for Technology and Innovation): Tailored nanostabilisers for biocomponent interfaces (TAINA, no: VTT-R-06743-08). In addition, Outi Liehunen, Riitta Isoniemi and Päivi Matikainen are thanked for the technical assistance.

Supplementary material

253_2013_4753_MOESM1_ESM.pdf (378 kb)
ESM 1 (PDF 378 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Outi Nivala
    • 1
    • 2
  • Maija-Liisa Mattinen
    • 2
  • Greta Faccio
    • 2
    • 3
  • Johanna Buchert
    • 2
  • Kristiina Kruus
    • 2
  1. 1.The Faculty of Biological and Environmental SciencesUniversity of HelsinkiHelsinkiFinland
  2. 2.VTT Technical Research Centre of FinlandVuorimiehentieFinland
  3. 3.Empa, Swiss Federal Laboratories for Materials Science and Technology - Laboratory for BiomaterialsSt. GallenSwitzerland

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