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Applied Microbiology and Biotechnology

, Volume 97, Issue 10, pp 4577–4587 | Cite as

Immobilization of nisin producer Lactococcus lactis strains to chitin with surface-displayed chitin-binding domain

  • Ömer ŞimşekEmail author
  • Seba Sabanoğlu
  • Ahmet Hilmi Çon
  • Nihat Karasu
  • Mustafa Akçelik
  • Per E. J. Saris
Applied microbial and cell physiology

Abstract

In this study, nisin producer Lactococcus lactis strains displaying cell surface chitin-binding domain (ChBD) and capable of immobilizing to chitin flakes were constructed. To obtain ChBD-based cell immobilization, Usp45 signal sequence with ChBD of chitinase A1 enzyme from Bacillus circulans was fused with different lengths of PrtP (153, 344, and 800 aa) or AcmA (242 aa) anchors derived from L. lactis. According to the whole cell ELISA analysis, ChBD was successfully expressed on the surface of L. lactis cells. Scanning electron microscope observations supported the conclusion of the binding analysis that L. lactis cells expressing the ChBD with long PrtP anchor (800 aa) did bind to chitin surfaces more efficiently than cells with the other ChBD anchors. The attained binding affinity of nisin producers for chitin flakes retained them in the fermentation during medium changes and enabled storage for sequential productions. Initial nisin production was stably maintained with many cycles. These results demonstrate that an efficient immobilization of L. lactis cells to chitin is possible for industrial scale repeated cycle or continuous nisin fermentation.

Keywords

Lactococcus lactis Nisin Chitin-binding domain Immobilization 

Notes

Acknowledgment

This work was supported by the Scientific and Technological Council of Turkey (TÜBİTAK) with the project 109 O 589.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Ömer Şimşek
    • 1
    Email author
  • Seba Sabanoğlu
    • 1
  • Ahmet Hilmi Çon
    • 2
  • Nihat Karasu
    • 1
  • Mustafa Akçelik
    • 3
  • Per E. J. Saris
    • 4
  1. 1.Department of Food Engineering, Faculty of EngineeringUniversity of PamukkaleDenizliTurkey
  2. 2.Department of Food Engineering, Faculty of EngineeringUniversity of Ondokuz MayisSamsunTurkey
  3. 3.Department of Biology, Faculty of ScienceUniversity of AnkaraAnkaraTurkey
  4. 4.Department of Food and Environmental SciencesUniversity of HelsinkiHelsinkiFinland

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