Applied Microbiology and Biotechnology

, Volume 97, Issue 9, pp 4075–4081 | Cite as

Quantitative analysis of growth and volatile fatty acid production by the anaerobic ruminal bacterium Megasphaera elsdenii T81

Applied microbial and cell physiology

Abstract

Megasphaera elsdenii T81 grew on either dl-lactate or d-glucose at similar rates (0.85 h−1) but displayed major differences in the fermentation of these substrates. Lactate was fermented at up to 210-mM concentration to yield acetic, propionic, butyric, and valeric acids. The bacterium was able to grow at much higher concentrations of d-glucose (500 mM), but never removed more than 80 mM of glucose from the medium, and nearly 60 % the glucose removed was sequestered as intracellular glycogen, with low yields of even-carbon acids (acetate, butyrate, caproate). In the presence of both substrates, glucose was not used until lactate was nearly exhausted, even by cells pregrown on glucose. Glucose-grown cultures maintained only low extracellular concentrations of acetate, and addition of exogenous acetate increased yields of butyrate, but not caproate. By contrast, exogenous acetate had little effect on lactate fermentation. At pH 6.6, growth rate was halved by exogenous addition of 60 mM propionate, 69 mM butyrate, 44 mM valerate, or 33 mM caproate; at pH 5.9, these values were reduced to 49, 49, 18, and 22 mM, respectively. The results are consistent with this species’ role as an effective ruminal lactate consumer and suggest that this organism may be useful for industrial production of volatile fatty acids from lactate if product tolerance could be improved. The poor fermentation of glucose and sensitivity to caproate suggests that this strain is not practical for industrial caproate production.

Keywords

Lactate Glucose Megasphaera elsdenii Volatile fatty acids 

Notes

Acknowledgments

We thank C.L. Odt for the technical assistance and M.B. Hall for the useful suggestions regarding glycogen analysis. This research was supported by USDA-ARS CRIS project 3655-41000-06-00D.

Conflict of interest

Mention of specific products is for informational purposes only and does not constitute an endorsement or warranty over similar products that may also be suitable.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012 (outside the USA) 2012

Authors and Affiliations

  1. 1.US Dairy Forage Research Center, Agricultural Research ServiceUnited States Department of AgricultureMadisonUSA
  2. 2.Department of BacteriologyUniversity of Wisconsin-MadisonMadisonUSA

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