Selective bactericidal activity of nanopatterned superhydrophobic cicada Psaltoda claripennis wing surfaces
- 2.2k Downloads
The nanopattern on the surface of Clanger cicada (Psaltoda claripennis) wings represents the first example of a new class of biomaterials that can kill bacteria on contact based solely on its physical surface structure. As such, they provide a model for the development of novel functional surfaces that possess an increased resistance to bacterial contamination and infection. Their effectiveness against a wide spectrum of bacteria, however, is yet to be established. Here, the bactericidal properties of the wings were tested against several bacterial species, possessing a range of combinations of morphology and cell wall type. The tested species were primarily pathogens, and included Bacillus subtilis, Branhamella catarrhalis, Escherichia coli, Planococcus maritimus, Pseudomonas aeruginosa, Pseudomonas fluorescens, and Staphylococcus aureus. The wings were found to consistently kill Gram-negative cells (i.e., B. catarrhalis, E. coli, P. aeruginosa, and P. fluorescens), while Gram-positive cells (B. subtilis, P. maritimus, and S. aureus) remained resistant. The morphology of the cells did not appear to play any role in determining cell susceptibility. The bactericidal activity of the wing was also found to be quite efficient; 6.1 ± 1.5 × 106 P. aeruginosa cells in suspension were inactivated per square centimeter of wing surface after 30-min incubation. These findings demonstrate the potential for the development of selective bactericidal surfaces incorporating cicada wing nanopatterns into the design.
KeywordsSelf-cleaning Nanopattern Bactericidal Insect wings Antibiofouling
This research was funded in part by the Advanced Manufacturing Cooperative Research Centre.
- Food and Drug Administration (FDA) (2009) Microbiological challenge testing, evaluation and definition of potentially hazardous foods. Accessed 25 Oct 2012.Google Scholar
- Food and Drug Administration (FDA), Center for Food Safety and Applied Nutrition (2001) The “Bad Bug Book” [Foodborne pathogenic microorganisms and natural toxins handbook]. http://www.cfsan.fda.gov/~mow/intro.html. Accessed 10 Dec 2001.
- Harris AD, Furuno JP, Roghmann M-C, Johnson JK, Conway LJ, Venezia RA, Standiford HC, Schweizer ML, Hebden JN, Moore AC, Perencevich EN (2010) Targeted surveillance of Methicillin-resistant Staphylococcus aureus and its potential use to guide empiric antibiotic therapy. Antimicrob Agents Chemother 54:3143–3148PubMedCrossRefGoogle Scholar
- Postgate JR (1969) Viable counts and viability. Methods Microbiol 1:611–628Google Scholar