Applied Microbiology and Biotechnology

, Volume 96, Issue 5, pp 1301–1312 | Cite as

Analysis of two distinct mycelial populations in liquid-grown Streptomyces cultures using a flow cytometry-based proteomics approach

  • G. Jerre van Veluw
  • Marloes L. C. Petrus
  • Jacob Gubbens
  • Richard de Graaf
  • Inez P. de Jong
  • Gilles P. van Wezel
  • Han A. B. Wösten
  • Dennis ClaessenEmail author
Genomics, transcriptomics, proteomics


Streptomycetes are proficient producers of enzymes and antibiotics. When grown in bioreactors, these filamentous microorganisms form mycelial pellets that consist of interconnected hyphae. We here employed a flow cytometry approach designed for large particles (COPAS) and demonstrate that liquid-grown Streptomyces cultures consist of two distinct populations of pellets. One population consists of mycelia with a constant mean diameter of approximately 260 μm, whereas the other population contains larger mycelia whose diameter depends on the strain, the age of the culture, and medium composition. Quantitative proteomics analysis revealed that 37 proteins differed in abundance between the two populations of pellets. Stress-related proteins and biosynthetic proteins for production of the calcium-dependent antibiotic were more abundant in the population of large mycelia, while proteins involved in DNA topology, modification, or degradation were overrepresented in the population of small mycelia. Deletion of genes for the cellulose synthase-like protein CslA and the chaplins affected the average size of the population of large pellets but not that of small pellets. Considering the fact that the production of enzymes and metabolites depends on pellet size, these results provide new leads toward rational strain design of Streptomyces strains tailored for industrial fermentations.


Heterogeneity Fermentation COPAS Cell wall Proteomics 



MLCP and DC were appointed at IBL from the award to Prof. Dr. P.J.J. Hooykaas as Academy Professor. Bogdan Florea and Hermen Overkleeft are thanked for help with mass spectrometry. HABW and GPvW gratefully acknowledge the Dutch Applied Research Council (STW) for continuing financial support

Supplementary material

253_2012_4490_MOESM1_ESM.pdf (187 kb)
ESM 1 (PDF 187 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • G. Jerre van Veluw
    • 1
  • Marloes L. C. Petrus
    • 2
    • 3
  • Jacob Gubbens
    • 4
  • Richard de Graaf
    • 2
    • 3
  • Inez P. de Jong
    • 2
    • 3
  • Gilles P. van Wezel
    • 2
  • Han A. B. Wösten
    • 1
  • Dennis Claessen
    • 2
    • 3
    Email author
  1. 1.Department of Microbiology, Institute of BiomembranesUniversity of UtrechtUtrechtThe Netherlands
  2. 2.Molecular Biotechnology, Institute Biology Leiden (IBL)Leiden UniversityLeidenThe Netherlands
  3. 3.Department of Molecular and Developmental Genetics, Institute Biology Leiden (IBL)Leiden UniversityLeidenThe Netherlands
  4. 4.Molecular Biotechnology, Leiden Institute of Chemistry (LIC)Leiden UniversityLeidenThe Netherlands

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