Applied Microbiology and Biotechnology

, Volume 97, Issue 13, pp 6077–6088 | Cite as

Bioremediation of the tobacco waste-contaminated soil by Pseudomonas sp. HF-1: nicotine degradation and microbial community analysis

  • Xin Wang
  • Lu Tang
  • Yanlai Yao
  • Haixia Wang
  • Hang Min
  • Zhenmei LuEmail author
Environmental biotechnology


The highly effective nicotine-degrading bacterium Pseudomonas sp. HF-1 was augmented into the tobacco waste-contaminated soil to degrade nicotine and evaluate the effect of the bioremediation. Comparing with non-adding (NA) systems, the treatments with addition of strain HF-1 (TA) exhibited considerably stronger pollution disposal abilities and higher stability of pH value and moisture content, especially in groups containing a large quantity of tobacco waste. The denaturing gradient gel electrophoresis (DGGE) profiles showed that the Shannon–Wiener index decreased with increasing wastes in the NA treatments, while a gradual increase was found in the TA groups. A comparison of sequences from DGGE bands demonstrated that there were differences in the dominant microbial species between the two treatments, suggesting that strain HF-1 could persist in the soil and enhance the efficiency of tobacco waste disposal. The results of real-time fluorescence quantitative PCR (RT-qPCR) also indicated that strain HF-1 existed in the TA systems and grew with relative high quantities. In conclusion, the nicotine-degrading strain HF-1 played a leading role in the bioremediation of the tobacco waste-contaminated soil and influenced the dynamics and structure of the microbial community.


Pseudomonas sp. HF-1 Tobacco waste-contaminated soil Bioremediation Microbial community Real-time qPCR 



This work was financially supported by the National Natural Science Foundation of China (no. 31170115, 31100032) and the Major Science and Technology Program for Water Pollution Control and Treatment (no. 2012ZX07101-012). We are sincerely grateful to Mr. Philip Alexzander Lorhrmann for greatly improving the use of English.

Supplementary material

253_2012_4433_MOESM1_ESM.pdf (545 kb)
ESM 1 (PDF 544 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Xin Wang
    • 1
  • Lu Tang
    • 1
  • Yanlai Yao
    • 2
  • Haixia Wang
    • 1
  • Hang Min
    • 1
  • Zhenmei Lu
    • 1
    Email author
  1. 1.Institute of Microbiology, College of Life ScienceZhejiang UniversityHangzhouChina
  2. 2.Institute of Environment, Resource, Soil and FertilizerZhejiang Academy of Agricultural SciencesHangzhouChina

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