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Applied Microbiology and Biotechnology

, Volume 97, Issue 6, pp 2483–2491 | Cite as

Cloning of a dibutyl phthalate hydrolase gene from Acinetobacter sp. strain M673 and functional analysis of its expression product in Escherichia coli

  • Jun WuEmail author
  • Xuewei Liao
  • Fangbo YuEmail author
  • Zhongbo Wei
  • Liuyan Yang
Biotechnologically relevant enzymes and proteins

Abstract

A dibutyl phthalate (DBP) transforming bacterium, strain M673, was isolated and identified as Acinetobacter sp. This strain could not grow on dialkyl phthalates, including dimethyl, diethyl, dipropyl, dibutyl, dipentyl, dihexyl, di(2-ethylhexyl), di-n-octyl, and dinonyl phthalate, but suspensions of cells could transform these compounds to phthalate via corresponding monoalkyl phthalates. During growth in Luria–Bertani medium, M673 produced the high amounts of non-DBP-induced intracellular hydrolase in the stationary phase. One DBP hydrolase gene containing an open reading frame of 1,095 bp was screened from a genomic library, and its expression product hydrolyzed various dialkyl phthalates to the corresponding monoalkyl phthalates.

Keywords

Dibutyl phthalate (DBP) Transformation Acinetobacter sp. DBP hydrolase gene 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation (Project nos. 21107047 and 31100087), the Fundamental Research Funds for Central Universities of The People’s Republic of China (Project nos. 1107021154 and 1118021114), and two grants (Project nos. Y3100018 and 2011C23065) from Zhejiang provincial government.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.State Key Laboratory of Pollution Control and Resource ReuseSchool of the Environment, Nanjing UniversityNanjingPeople’s Republic of China
  2. 2.Center for Analysis and Testing, Nanjing Normal UniversityNanjingPeople’s Republic of China
  3. 3.Department of Environmental Biology, School of the EnvironmentNanjing UniversityNanjingPeople’s Republic of China
  4. 4.Department of Environmental Sciences, School of Environment and Resource SciencesZhejiang Agriculture and Forestry UniversityLinanPeople’s Republic of China

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