Applied Microbiology and Biotechnology

, Volume 96, Issue 5, pp 1209–1216 | Cite as

Microbial production of itaconic acid: developing a stable platform for high product concentrations

  • Anja Kuenz
  • Yvonne Gallenmüller
  • Thomas Willke
  • Klaus-Dieter Vorlop
Biotechnological products and process engineering


Biotechnologically produced itaconic acid (IA) is a promising organic acid with a wide range of applications and the potential to open up new application fields in the area of polymer chemistry, pharmacy, and agriculture. In this study, a systematic process optimization was performed with an own isolated strain of Aspergillus terreus and transferred from a 250-mL to a 15-L scale. An IA concentration of 86.2 g/L was achieved within 7 days with an overall productivity of 0.51 g/(L h), a maximum productivity of 1.2 g/(L h), and a yield of 86 mol%. A cultivation of other well-known A. terreus strains with the developed process showed no significant differences. Based on this, a process is developed providing a high final IA concentration independent of the used strain combined with high reproducibility.


Aspergillus terreus Itaconic acid Optimization Submerse cultivation Scale up 



Many thanks for the financial support by the Fachagentur Nachwachsende Rohstoffe e.V. (grant no. 22008203) and the Südzucker AG.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Anja Kuenz
    • 1
  • Yvonne Gallenmüller
    • 1
  • Thomas Willke
    • 1
  • Klaus-Dieter Vorlop
    • 1
  1. 1.vTI—Institute of Agricultural Technology and Biosystems EngineeringBraunschweigGermany

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