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Applied Microbiology and Biotechnology

, Volume 96, Issue 6, pp 1561–1576 | Cite as

Characterization of Bifidobacterium spp. strains for the treatment of enteric disorders in newborns

  • Irene Aloisio
  • Cecilia Santini
  • Bruno Biavati
  • Giovanni Dinelli
  • Avrelija Cencič
  • Walter Chingwaru
  • Luca Mogna
  • Diana Di GioiaEmail author
Applied microbial and cell physiology

Abstract

Several studies support the use of probiotics for the treatment of minor gastrointestinal problems in infants. Positive effects on newborn colics have been evidenced after administration of Lactobacillus strains, whereas no studies have been reported regarding the use of bifidobacteria for this purpose. This work was therefore aimed at the characterization of Bifidobacterium strains capable of inhibiting the growth of pathogens typical of the infant gastrointestinal tract and of coliforms isolated from colic newborns. Among the 46 Bifidobacterium strains considered, 16 showed high antimicrobial activity against potential pathogens; these strains were further characterized from a taxonomic point of view, for the presence and transferability of antibiotic resistances, for citotoxic effects and adhesion to nontumorigenic gut epithelium cell lines. Moreover, their ability to stimulate gut health by increasing the metabolic activity and the immune response of epithelial cells was also studied. The examination of all these features allowed to identify three Bifidobacterium breve strains and a Bifidobacterium longum subsp. longum strain as potential probiotics for the treatments of enteric disorders in newborns such as infantile colics. A validation clinical trial involving the selected strains is being planned.

Keywords

Bifidobacterium Probiotics Newborns Enteric disorders Nontumorigenic cell lines 

Notes

Aknowledgments

The study was funded by the University of Bologna, Program RFO number J61J10000790001, assigned to Prof. Bruno Biavati. The authors are grateful to Dr. Giovanni Mogna and Dr. Paolo Strozzi of Probiotical SpA for fruitful discussions.

Supplementary material

253_2012_4138_MOESM1_ESM.pdf (16 kb)
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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Irene Aloisio
    • 1
  • Cecilia Santini
    • 1
  • Bruno Biavati
    • 1
  • Giovanni Dinelli
    • 1
  • Avrelija Cencič
    • 2
    • 3
  • Walter Chingwaru
    • 2
  • Luca Mogna
    • 4
  • Diana Di Gioia
    • 1
    Email author
  1. 1.Department of Agroenvironmental Sciences and TechnologiesUniversity of BolognaBolognaItaly
  2. 2.Department of Microbiology, Biochemistry, and Biotechnology, Faculty of Agriculture and Life SciencesUniversity of MariborHočeSlovenia
  3. 3.Department of Biochemistry and Nutrition, Faculty of MedicineUniversity of MariborMariborSlovenia
  4. 4.Probiotical SpANovaraItaly

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