Applied Microbiology and Biotechnology

, Volume 97, Issue 6, pp 2725–2733

Denitrification performance and microbial diversity in a packed-bed bioreactor using PCL as carbon source and biofilm carrier

Environmental biotechnology

DOI: 10.1007/s00253-012-4110-4

Cite this article as:
Wu, W., Yang, L. & Wang, J. Appl Microbiol Biotechnol (2013) 97: 2725. doi:10.1007/s00253-012-4110-4


Polycaprolactone (PCL) was used as both carbon source and biofilm support for denitrifying bacteria in a packed-bed bioreactor. The denitrification performance and microbial diversity were investigated. The microbial community of biofilm developed on the surface of PCL in the reactor was analyzed by pyrosequencing method. The experimental results showed the average nitrate removal efficiency reached 93 % at stable operation. ESEM observation and FTIR analysis were conducted to characterize the PCL structure before and after microbial utilization. For the microbial community, Betaproteobacteria predominated, and most of the PCL-degrading denitrifying bacteria assigned to the family of Comamonadacea. Denitrifying bacteria accounted for more than 20 % in the total population, indicating that PCL is a good carrier and carbon source for biological denitrification.


Nitrate Denitrification PCL Solid carbon source Pyrosequencing Betaproteobacteria 

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.College of Environmental Sciences and EngineeringPeking UniversityBeijingPeople’s Republic of China
  2. 2.Laboratory of Environmental Technology, INETTsinghua UniversityBeijingPeople’s Republic of China

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