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Applied Microbiology and Biotechnology

, Volume 94, Issue 6, pp 1423–1447 | Cite as

Microbial steroid transformations: current state and prospects

  • Marina V. Donova
  • Olga V. Egorova
Mini-Review

Abstract

Studies of steroid modifications catalyzed by microbial whole cells represent a well-established research area in white biotechnology. Still, advances over the last decade in genetic and metabolic engineering, whole-cell biocatalysis in non-conventional media, and process monitoring raised research in this field to a new level. This review summarizes the data on microbial steroid conversion obtained since 2003. The key reactions of structural steroid functionalization by microorganisms are highlighted including sterol side-chain degradation, hydroxylation at various positions of the steroid core, and redox reactions. We also describe methods for enhancement of bioprocess productivity, selectivity of target reactions, and application of microbial transformations for production of valuable pharmaceutical ingredients and precursors. Challenges and prospects of whole-cell biocatalysis applications in steroid industry are discussed.

Keywords

Steroid Microbial transformation Bioconversion Sterol Side-chain degradation Hydroxylation Dehydrogenation Sterol catabolism Whole-cell biocatalysis 

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.G.K. Skryabin Institute of Biochemistry and Physiology of MicroorganismsRussian Academy of SciencesMoscow RegionRussia

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