Applied Microbiology and Biotechnology

, Volume 94, Issue 5, pp 1279–1287

Characterization of Lactobacillus salivarius CECT 5713, a strain isolated from human milk: from genotype to phenotype

  • Susana Langa
  • Antonio Maldonado-Barragán
  • Susana Delgado
  • Rebeca Martín
  • Virginia Martín
  • Esther Jiménez
  • José L. Ruíz-Barba
  • Baltasar Mayo
  • Ruth I. Connor
  • Juan Evaristo Suárez
  • Juan M. Rodríguez
Genomics, transcriptomics, proteomics

Abstract

Lactobacillus salivarius CECT 5713, isolated from human milk, has immunomodulatory, anti-inflammatory and antiinfectious properties, as revealed by several in vitro and in vivo assays, which suggests a strong potential as a probiotic strain. In this work, the relationships between several genetic features of L. salivarius CECT 5713 and the corresponding phenotypes were evaluated. Although it contains a plasmid-encoded bacteriocin cluster, no bacteriocin biosynthesis was observed, possibly due to a 4-bp deletion at the beginning of the histidine kinase determinant abpK. The genome of L. salivarius CECT 5713 harbours two apparently complete prophages of 39.6 and 48 kbp. Upon induction, the 48-kbp prophage became liberated from the bacterial genome, but no DNA replication took place, which resulted in lysis of the cultures but not in phage progeny generation. The strain was sensitive to most antibiotics tested and no transmissible genes potentially involved in antibiotic resistance were detected. Finally, the genome of L. salivarius CECT 5713 contained four ORFs potentially involved in human molecular mimetism. Among them, protein 1230 was considered of particular relevance because of its similarity with dendritic cell-related proteins. Subsequently, in vitro assays revealed the ability of L. salivarius CECT 5713 to stimulate the maturation of immature dendritic cells and to inhibit the in vitro infectivity of HIV-1.

Keywords

Lactobacillus salivarius Probiotic Genome Phage Antibiotic resistance Bacteriocin Dendritic cell HIV 

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Susana Langa
    • 1
    • 2
  • Antonio Maldonado-Barragán
    • 3
  • Susana Delgado
    • 4
  • Rebeca Martín
    • 5
  • Virginia Martín
    • 1
  • Esther Jiménez
    • 1
  • José L. Ruíz-Barba
    • 3
  • Baltasar Mayo
    • 4
  • Ruth I. Connor
    • 6
  • Juan Evaristo Suárez
    • 4
    • 5
  • Juan M. Rodríguez
    • 1
  1. 1.Departamento de Nutrición, Bromatología y Tecnología de los AlimentosUniversidad Complutense de MadridMadridSpain
  2. 2.Departamento de Tecnología de AlimentosInstituto Nacional de Investigación y Tecnología Agraria y AlimentariaMadridSpain
  3. 3.Departamento de Biotecnología de AlimentosInstituto de la Grasa-CSICSevillaSpain
  4. 4.Instituto de Productos Lácteos de Asturias (IPLA-CSIC)Carretera de Infiesto s/nVillaviciosaSpain
  5. 5.Area de Microbiología e Instituto de BiotecnologíaUniversidad de OviedoOviedoSpain
  6. 6.Department of Microbiology and ImmunologyDartmouth Medical SchoolLebanonUSA

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