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Applied Microbiology and Biotechnology

, Volume 97, Issue 2, pp 881–889 | Cite as

Effective bioremoval and detoxification of textile dye mixture by Alishewanella sp. KMK6

  • Yogesh M. Kolekar
  • Pallavi D. Konde
  • Vijay L. Markad
  • Sharwari V. Kulkarni
  • Ashvini U. Chaudhari
  • Kisan M. KodamEmail author
Environmental biotechnology

Abstract

Alishewanella sp. strain KMK6 was able to degrade mixture of textile dyes (0.5–2.0 g l−1) within 8 h. An initial 28 % reduction in COD was observed immediately after decolorization at static anoxic conditions which on further incubation at shaking conditions reduced by 90 %. Partially purified azoreductase was able to utilize different azo dyes as substrates. The HPLC profile of dye degradation showed formation of metabolic products. Further FTIR analysis showed significant changes in the peaks corresponding to functional groups present in dye mixture and its degradation products. The genotoxicity assessment showed that the dye degradation products were non-toxic compared to dye mixture.

Keywords

Alishewanella sp. KMK6 Azoreductase Biodegradation Azo dyes COD 

Notes

Acknowledgment

The authors, YMK and VLM, thank the University Grants Commission (UGC), New Delhi, India for research fellowship.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Yogesh M. Kolekar
    • 1
  • Pallavi D. Konde
    • 1
  • Vijay L. Markad
    • 1
  • Sharwari V. Kulkarni
    • 1
  • Ashvini U. Chaudhari
    • 1
  • Kisan M. Kodam
    • 1
    Email author
  1. 1.Biochemistry Division, Department of ChemistryUniversity of PunePuneIndia

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