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Applied Microbiology and Biotechnology

, Volume 97, Issue 2, pp 661–672 | Cite as

Oxidation of phenolic compounds by the bifunctional catalase–phenol oxidase (CATPO) from Scytalidium thermophilum

  • Gulden Koclar AvciEmail author
  • Nursen Coruh
  • Ufuk Bolukbasi
  • Zumrut B. Ogel
Biotechnologically relevant enzymes and proteins

Abstract

The thermophilic fungus Scytalidium thermophilum produces a novel bifunctional catalase with an additional phenol oxidase activity (CATPO); however, its phenol oxidation spectrum is not known. Here, 14 phenolic compounds were selected as substrates, among which (+)-catechin, catechol, caffeic acid, and chlorogenic acid yielded distinct oxidation products examined by reversed-phase HPLC chromatography method. Characterization of the products by LC-ESI/MS and UV–vis spectroscopy suggests the formation of dimers of dehydrocatechin type B (hydrophilic) and type A (hydrophobic), as well as oligomers, namely, a trimer and tetramer from (+)-catechin, the formation of a dimer and oligomer of catechol, a dimer from caffeic acid with a caffeicin-like structure, as well as trimeric and tetrameric derivatives, and a single major product from chlorogenic acid suggested to be a dimer. Based on the results, CATPO oxidizes phenolic compounds ranging from simple phenols to polyphenols but all having an ortho-diphenolic structure in common. The enzyme also appears to have stereoselectivity due to the oxidation of (+)-catechin, but not that of epicatechin. It is suggested that CATPO may contribute to the antioxidant mechanism of the fungus and may be of value for future food and biotechnology applications where such a bifunctional activity would be desirable.

Keywords

Scytalidium thermophilum Catalase Phenol oxidase Phenolic compounds Oxidation Antioxidant 

Notes

Acknowledgements

We would like to thank TUBITAK (TBAG-107 T611) and Middle East Technical University (BAP-07-02-2010-101) for financial support.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Gulden Koclar Avci
    • 1
    Email author
  • Nursen Coruh
    • 2
  • Ufuk Bolukbasi
    • 3
  • Zumrut B. Ogel
    • 4
  1. 1.Department of BiotechnologyMiddle East Technical UniversityAnkaraTurkey
  2. 2.Department of ChemistryMiddle East Technical UniversityAnkaraTurkey
  3. 3.Department of Chemical EngineeringMiddle East Technical UniversityAnkaraTurkey
  4. 4.Department of Food EngineeringMiddle East Technical UniversityAnkaraTurkey

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