Applied Microbiology and Biotechnology

, Volume 94, Issue 4, pp 939–948

Effect of pretreatment of hydrothermally processed rice straw with laccase-displaying yeast on ethanol fermentation

  • Akihito Nakanishi
  • Jun Gu Bae
  • Kotaro Fukai
  • Naoki Tokumoto
  • Kouichi Kuroda
  • Jun Ogawa
  • Masato Nakatani
  • Sakayu Shimizu
  • Mitsuyoshi Ueda
Biotechnological products and process engineering

Abstract

A gene encoding laccase I was identified and cloned from the white-rot fungus Trametes sp. Ha1. Laccase I contained 10 introns and an original secretion signal sequence. After laccase I without introns was prepared by overlapping polymerase chain reaction, it was inserted into expression vector pULD1 for yeast cell surface display. The oxidation activity of a laccase-I-displaying yeast as a whole-cell biocatalyst was examined with 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), and the constructed yeast showed a high oxidation activity. After the pretreatment of hydrothermally processed rice straw (HPRS) with laccase-I-displaying yeast with ABTS, fermentation was conducted with yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase with HPRS. Fermentation of HPRS treated with laccase-I-displaying yeast was performed with 1.21-fold higher activities than those of HPRS treated with control yeast. The results indicated that pretreatment with laccase-I-displaying yeast with ABTS was effective for direct fermentation of cellulosic materials by yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase.

Keywords

Biorefinery Laccase Cell surface engineering of yeast Trametes sp. Lignin 

Supplementary material

253_2012_3876_MOESM1_ESM.doc (189 kb)
ESM 1(DOC 189 kb)

References

  1. Abe S, Takagi M (1991) Simultaneous saccharification and fermentation of cellulose to lactic acid. Biotechnol Bioeng 37:93–96CrossRefGoogle Scholar
  2. Baldrian P (2006) Fungal laccases—occurrence and properties. FEMS Microbiol Rev 30:215–242CrossRefGoogle Scholar
  3. Bulter T, Alcalde M, Sieber V, Meinhold P, Schlachtbauer C, Arnold FH (2003) Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution. Appl Environ Microbiol 69:987–995CrossRefGoogle Scholar
  4. Childs RE, Bardsley WG (1975) The steady-state kinetics of peroxidase with 2,2′-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid) as chromogen. J Biochem 145:93–103Google Scholar
  5. Fan F, Zhuo R, Sun S, Wan X, Jiang M, Zhang X, Yant Y (2011) Cloning and functional analysis of a new laccase gene from Trametes sp. 48424 which had the high yield of laccase and strong ability for decolorizing different dyes. Bioresour Technol 102:3126–3137CrossRefGoogle Scholar
  6. Fujita Y, Takahashi S, Ueda M, Tanaka A, Okada H, Morikawa Y, Kawaguchi T, Arai M, Fukuda H, Kondo A (2002) Direct and efficient production of ethanol from cellulosic material with a yeast strain displaying cellulolytic enzymes. Appl Environ Microbiol 68:5136–5141CrossRefGoogle Scholar
  7. Fujita Y, Ito J, Ueda M, Fukuda H, Kondo A (2004) Synergistic saccharification, and direct fermentation to ethanol, of amorphous cellulose by use of an engineered yeast strain codisplaying three types of cellulolytic enzyme. Appl Environ Microbiol 70:1207–1212CrossRefGoogle Scholar
  8. Gerngross TU, Slater SC (2000) How green are green plastics? Sci Am 283:37–41CrossRefGoogle Scholar
  9. Herpöel I, Jeller H, Fang G, Petit-Conil M, Bourbonnais R, Robert JL, Asther M, Sigoillot JC (2002) Efficient enzymatic delignification of wheat straw pulp by a sequential xylanase-laccase mediator treatment. J Pulp Pap Sci 28:67–71Google Scholar
  10. Hoshida H, Nakao M, Kanazawa H, Kubo K, Hakukawa T, Morimasa K, Akada R, Nishizawa Y (2001) Isolation of five laccase gene sequences from the white-rot fungus Trametes sanguinea by PCR, and cloning, characterization and expression of the laccase cDNA in yeasts. J Biosci Bioeng 92:372–380Google Scholar
  11. Ibarra D, Romero J, Martinez MJ, Martinez AT, Camarero S (2006) Exploring the enzymatic parameters for optimal delignification of eucalypt pulp by laccase-mediator. Enzyme Microb Technol 39:1319–1327CrossRefGoogle Scholar
  12. Ito H, Fukuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cation. J Bacteriol 153:163–168Google Scholar
  13. Jeon SD, Yu KO, Kim SW, Han SO (2011) A celluloytic complex from Clostridium cellulovorans consisting of mannanase B and endoglucanase E has synergistic effects on galactomannan degradation. Appl Microbiol Biotechnol 90:565–572CrossRefGoogle Scholar
  14. Kamm B, Kamm M (2004) Principles of biorefineries. Appl Microbiol Biotechnol 64:137–145CrossRefGoogle Scholar
  15. Kobori H, Sato M, Osumi M (1992) Relationship of actin organization to growth in the two forms of the dimorphic yeast Candida tropicalis. Protoplasma 167:193–204CrossRefGoogle Scholar
  16. Kojima Y, Tsukuda Y, Kawai Y, Tsukamoto A, Sugiura J, Sakaino M, Kita Y (1990) Cloning, sequence analysis, and expression of ligninolytic phenoloxidase genes of the white-rot basidiomycete Coriolus hirsutus. J Biol Chem 265:15224–15230Google Scholar
  17. Kondo A, Ueda M (2004) Yeast cell-surface display—applications of molecular display. Appl Microbiol Biotechnol 64:28–40CrossRefGoogle Scholar
  18. Kotaka A, Sahara H, Kuroda K, Ueda M, Hata Y (2010) Enhancement of β-glucosidase activity on the cell-surface of sake yeast by disruption of SED1. J Biosci Bioeng 109:442–446CrossRefGoogle Scholar
  19. Kuroda K, Matsui K, Higuchi S, Kotaka A, Sahara H, Hata Y, Ueda M (2009) Enhancement of display efficiency in yeast display system by vector engineering and gene disruption. Appl Microbiol Biotechnol 82:713–719CrossRefGoogle Scholar
  20. Labat GA, Gonçalves AR (2008) Oxidation in acidic medium of lignins from agricultural residues. Appl Biochem Biotechnol 148:151–161CrossRefGoogle Scholar
  21. Lebrun JD, Lamy I, Mougin C (2011) Favouring the bioavailability of Zn and Cu to enhance the production of lignin-modifying enzymes in Trametes versicolor cultures. Bioresour Technol 102:3103–3109CrossRefGoogle Scholar
  22. Mohamad SB, Ong AL, Ripen AM (2008) Evolutionary trace analysis at the ligand binding site of laccase. Bioinformation 2:369–372CrossRefGoogle Scholar
  23. Murai T, Ueda M, Kawaguchi T, Arai M, Tanaka A (1998) Assimilation of cellooligosaccharides by a cell surface-engineered yeast expressing β-glucosidase and carboxymethylcellulose from Aspergillus aculeatus. Appl Environ Microbiol 64:4857–4861Google Scholar
  24. Nakatani M, Hibi M, Minoda M, Ogawa J, Yokozeki K, Shimizu S (2010) Two laccase isoenzymes and a peroxidase of a commercial laccase-producing basidiomycete, Trametes sp. Ha1. N Biotechnol 27:317–323CrossRefGoogle Scholar
  25. Octave S, Thomas D (2009) Biorefinery: toward an industrial metabolism. Biochimie 91:659–664CrossRefGoogle Scholar
  26. Park YS, Jeong HS, Sung HC, Yun CW (2005) Sed1p interacts with Arn3p physically and mediates ferrioxamine B uptake in Saccharomyces cerevisiae. Curr Genet 47:150–155CrossRefGoogle Scholar
  27. Shibasaki S, Ueda M, Iizuka T, Hirayama M, Ikeda Y, Kamasawa N, Osumi M, Tanaka A (2001) Quantitative evaluation of the enhanced green fluorescent protein displayed on the cell surface of Saccharomyces cerevisiae by the fluorometric and confocal laser scanning microscopic analysis. Appl Microbiol Biotechnol 55:471–475CrossRefGoogle Scholar
  28. Shimizu S, Nakatani M (1996) Laccase and its production. Patent JP 8:070861Google Scholar
  29. Sigoillot C, Record E, Belle V, Robert JL, Levasseur A, Punt PJ, van den Hondel CA, Fournel A, Sigoillot JC, Asther M (2004) Natural and recombinant fungal laccases for paper pulp bleaching. Appl Microbiol Biotechnol 64:346–352CrossRefGoogle Scholar
  30. Sinha AK, Sharma UK, Sharma N (2008) A comprehensive review on vanilla flavor: extraction, isolation and quantification of vanillin and others constituents. Int J Food Sci Nutr 59:299–326CrossRefGoogle Scholar
  31. Smith M, Shnyreva A, Wood DA, Thurston CF (1998) Tandem organization and highly disparate expression of the two laccase genes lcc1 and lcc2 in the cultivated mushroom Agaricus bisporus. Microbiology 144:1063–1069CrossRefGoogle Scholar
  32. Sulistyaningdyah WT, Ogawa J, Tanaka H, Maeda C, Shimizu S (2004) Characterization of alkaliphilic laccase activity in the culture supernatant of Myrothecium verrucaria 24G-4 in comparison with bilirubin oxidase. FEMS Microbiol Lett 230:209–214CrossRefGoogle Scholar
  33. Xiao YZ, Tu XM, Wang J, Zhang M, Cheng Q, Zeng WY, Shi YY (2003) Purification, molecular characterization and reactivity with aromatic compounds of a laccase from basidiomycete Trametes sp. strain AH28-2. Appl Microbiol Biotechnol 60:700–707Google Scholar
  34. Xiao YZ, Hong YZ, Li JF, Hang J, Tong PG, Fang W, Zhou CZ (2006) Cloning of novel laccase isozyme genes from Trametes sp. AH28-2 and analyses of their differential expression. Appl Microbiol Biotechnol 71:493–501CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Akihito Nakanishi
    • 1
  • Jun Gu Bae
    • 1
  • Kotaro Fukai
    • 1
  • Naoki Tokumoto
    • 1
  • Kouichi Kuroda
    • 1
  • Jun Ogawa
    • 1
  • Masato Nakatani
    • 2
  • Sakayu Shimizu
    • 1
  • Mitsuyoshi Ueda
    • 1
  1. 1.Division of Applied Life Sciences, Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.Daiwa KaseiShigaJapan

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