Ixr1p and the control of the Saccharomyces cerevisiae hypoxic response
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In Saccharomyces cerevisiae, adaptation to hypoxia/anaerobiosis requires the transcriptional induction or derepression of multiple genes organized in regulons controlled by specific transcriptional regulators. Ixr1p is a transcriptional regulatory factor that causes aerobic repression of several hypoxic genes (COX5B, TIR1, and HEM13) and also the activation of HEM13 during hypoxic growth. Analysis of the transcriptome of the wild-type strain BY4741 and its isogenic derivative Δixr1, grown in aerobic and hypoxic conditions, reveals differential regulation of genes related not only to the hypoxic and oxidative stress responses but also to the re-adaptation of catabolic and anabolic fluxes in response to oxygen limitation. The function of Ixr1p in the transcriptional regulation of genes from the sulfate assimilation pathway and other pathways producing α-keto acids is of biotechnological importance for industries based on yeast-derived fermentation products.
KeywordsTranscriptional regulation Oxidative stress Sulfate assimilation pathway
This research was supported by grants BFU2006-03961 and BFU2009-08854 from MICINN (Spain), co-financed by FEDER (CEE). General support to the laboratory during 2008-11 was funded by Xunta de Galicia (Consolidación C.E.O.U.2008/008), co-financed by FEDER. A.V’s salary was funded by the “Lucas-Labrada program-2008” from Xunta de Galicia.
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