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Applied Microbiology and Biotechnology

, Volume 93, Issue 5, pp 1937–1945 | Cite as

Immunological features and the ability of inhibitory effects on enzymatic activity of an epitope vaccine composed of cholera toxin B subunit and B cell epitope from Helicobacter pylori urease A subunit

  • Le Guo
  • Xiaokang Li
  • Feng Tang
  • Yunmian He
  • Yingying Xing
  • Xuepeng Deng
  • Tao XiEmail author
Biotechnological products and process engineering

Abstract

Epitope vaccine based on urease of Helicobacter pylori is a promising option for prophylactic and therapeutic vaccination against H. pylori infection. In this study, we constructed an epitope vaccine with mucosal adjuvant cholera toxin B subunit (CTB) and an epitope (UreA183-203) of H. pylori urease A subunit named CTB-UA. The CTB-UA fusion protein was expressed in Escherichia coli, and the purified protein was used for intraperitoneal immunization experiments in BALB/c mice. The experimental results indicated that anti-CTB-UA antibody could recognize both H. pylori urease A subunit (UreA) and urease B subunit (UreB). Besides, the CTB-UA epitope vaccine had good immunogenicity and immunoreactivity and could induce specific neutralizing antibodies which showed effectively inhibitory effect on the enzymatic activity of H. pylori urease. CTB-UA is a promising molecule to be investigated as H. pylori vaccine antigen candidate.

Keywords

Epitope vaccine Helicobacter pylori Cholera toxin B subunit Urease A subunit Neutralizing antibody 

Notes

Acknowledgments

This work was supported by the Science Foundation of China Pharmaceutical University (grant no, JKY2009023) and Postgraduate Innovation Project of Jiangsu Province (grant no, CXZZ11_0817). We thank especially Professor Wutong Wu for his contributions in implementation of this experiment.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Le Guo
    • 1
  • Xiaokang Li
    • 1
  • Feng Tang
    • 1
  • Yunmian He
    • 1
  • Yingying Xing
    • 1
  • Xuepeng Deng
    • 1
  • Tao Xi
    • 1
    Email author
  1. 1.Biotechnology Center, School of Life Science and TechnologyChina Pharmaceutical UniversityNanjingChina

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