Applied Microbiology and Biotechnology

, Volume 95, Issue 1, pp 255–262

Isolation and characterization of a Klebsiella oxytoca strain for simultaneous azo-dye anaerobic reduction and bio-hydrogen production

  • Lei Yu
  • Wen-Wei Li
  • Michael Hon-Wah Lam
  • Han-Qing Yu
  • Chao Wu
Environmental biotechnology

Abstract

A facultative anaerobic bacteria strain GS-4-08, isolated from an anaerobic sequence batch reactor for synthetic dye wastewater treatment, was investigated for azo-dye decolorization. This bacterium was identified as a member of Klebsiella oxytoca based on Gram staining, morphology characterization and 16S rRNA gene analysis. It exhibited a good capacity of simultaneous decolorization and hydrogen production in the presence of electron donor. The hydrogen production was less affected even at a high Methyl Orange (MO) concentration of 0.5 mM, indicating a superior tolerability of this strain to MO. This efficient bio-hydrogen production from electron donor can not only avoid bacterial inhibition due to accumulation of volatile fatty acids during MO decolorization, but also can recover considerable energy from dye wastewater.

Keywords

Anaerobic Bio-hydrogen Decolorization Electron donor Klebisiella oxytoca Methyl Orange (MO) 

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Lei Yu
    • 1
    • 2
    • 3
  • Wen-Wei Li
    • 1
    • 2
  • Michael Hon-Wah Lam
    • 1
    • 3
  • Han-Qing Yu
    • 2
  • Chao Wu
    • 2
  1. 1.Advanced Laboratory for Environmental Research and TechnologySuzhouChina
  2. 2.Department of ChemistryUniversity of Science and Technology of ChinaHefeiChina
  3. 3.Department of Biology and ChemistryCity University of Hong KongKowloonHong Kong

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