Advertisement

Applied Microbiology and Biotechnology

, Volume 94, Issue 2, pp 505–515 | Cite as

Bioremediation of β-cypermethrin and 3-phenoxybenzaldehyde contaminated soils using Streptomyces aureus HP-S-01

  • Shaohua Chen
  • Peng Geng
  • Ying Xiao
  • Meiying HuEmail author
Environmental biotechnology

Abstract

Using laboratory and field experiments, the ability of Streptomyces aureus HP-S-01 to eliminate β-cypermethrin (β-CP) and its metabolite 3-phenoxybenzaldehyde (3-PBA) in soils was investigated. In the laboratory, 80.5% and 73.1% of the initial dose of β-CP and 3-PBA (50 mg kg−1) was removed in sterilized soils within 10 days, respectively, while in the same period, disappearance rate of β-CP and 3-PBA in non-sterilized soils was higher and reached 87.8% and 79.3%, respectively. Furthermore, the disappearance process followed the first-order kinetics and the half-life (T 1/2) for β-CP and 3-PBA reduced by 20.3–52.9 and 133.7–186.8 days, respectively, as compared to the controls. The addition of sucrose to the soils enhanced the ability of strain HP-S-01 to eliminate β-CP and 3-PBA. Similar results were observed in the field experiments. The introduced strain HP-S-01 quickly adapted to the environment and rapidly removed β-CP and 3-PBA without any lag phases in the field experiments. Compared with the controls, 47.9% and 67.0% of applied dose of β-CP and 3-PBA was removed from the soils without extra carbon sources and 52.5% and 73.3% of β-CP and 3-PBA was eliminated in soils supplemented with sucrose within 10 days, respectively. Analysis of β-CP degradation products in soil indicated that the tested strain transform β-CP to 3-PBA and α-hydroxy-3-phenoxy-benzeneacetonitrile. However, both intermediates were transient and they disappeared after 10 days. Therefore, the selected actinomyces strain HP-S-01 is suitable for the efficient and rapid bioremediation of β-CP contaminated soils.

Keywords

β-Cypermethrin 3-Phenoxybenzaldehyde Bioremediation Streptomyces aureus HP-S-01 Kinetics Soil 

Notes

Acknowledgments

We gratefully acknowledge the grants from the National Natural Science Foundation (no. 30871660) and Project of Scientific Technological Planning of Guangdong Province (no. 2009B020310005), People’s Republic of China.

References

  1. Ansari AR, Rahman S, Kaur M, Anjum S, Raisuddin S (2011) In vivo cytogenetic and oxidative stress-inducing effects of cypermethrin in freshwater fish, Channa punctata Bloch. Ecotoxicol Environ Saf 74:150–156CrossRefGoogle Scholar
  2. Anwar S, Liaquat F, Khan QM, Khalid ZM, Iqbal S (2009) Biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-trichloro-2-pyridinolby Bacillus pumilus strain C2A1. J Hazard Mater 168:400–405CrossRefGoogle Scholar
  3. Boucard TK, McNeill C, Bardgett RD, Paynter CD, Semple TK (2008) The impact of synthetic pyrethroid and organophosphate sheep dip formulations on microbial activity in soil. Environ Pollut 153:207–214CrossRefGoogle Scholar
  4. Budd R, Bondarenko S, Haver DL, Kabashima JN, Gan JY (2007) Occurrence and bioavailability of pyrethroids in a mixed land use watershed. J Environ Qual 36:1006–1012CrossRefGoogle Scholar
  5. Chen SH, Lai KP, Li YN, Hu MY, Zhang YB, Zeng Y (2011a) Biodegradation of deltamethrin and its hydrolysis product 3-phenoxybenzaldehyde by a newly isolated Streptomyces aureus strain HP-S-01. Appl Microbiol Biotechnol 90:1471–1483CrossRefGoogle Scholar
  6. Chen SH, Hu QB, Hu MY, Luo JJ, Weng QF, Lai KP (2011b) Isolation and characterization of a fungus able to degrade pyrethroids and 3-phenoxybenzaldehyde. Bioresource Technol 102:8110–8116CrossRefGoogle Scholar
  7. Chen SH, Hu MY, Liu JJ, Zhong GH, Yang L, Rizwan-ul-Haq M, Han HT (2011c) Biodegradation of beta-cypermethrin and 3-phenoxybenzoic acid by a novel Ochrobactrum lupini DG-S-01. J Hazard Mater 187:433–440CrossRefGoogle Scholar
  8. Chen SH, Yang L, Hu MY, Liu JJ (2011d) Biodegradation of fenvalerate and 3-phenoxybenzoic acid by a novel Stenotrophomonas sp. strain ZS-S-01 and its use in bioremediation of contaminated soils. Appl Microbiol Biotechnol 90:755–767CrossRefGoogle Scholar
  9. Cycoń M, Wojcik M, Piotrowska-Seget Z (2009) Biodegradation of the organophosphorus insecticide diazinon by Serratia sp. and Pseudomonas sp. and their use in bioremediation of contaminated soil. Chemosphere 76:494–501CrossRefGoogle Scholar
  10. Cycoń M, Wojcik M, Piotrowska-Seget Z, Kozdroj J (2010) Responses of indigenous microorganisms to a fungicidal mixture of mancozeb and dimethomorph added to sandy soils. Int Biodeterior Biodegrad 64:316–323CrossRefGoogle Scholar
  11. Cycoń M, Wojcik M, Piotrowska-Seget Z (2011) Biodegradation kinetics of the benzimidazole fungicide thiophanate-methyl by bacteria isolated from loamy sand soil. Biodegradation 22:573–583CrossRefGoogle Scholar
  12. Delgado-Moreno L, Wu L, Gan J (2010) Effect of dissolved organic carbon on sorption of pyrethroids to sediments. Environ Sci Technol 44:8473–8478CrossRefGoogle Scholar
  13. Duan XQ, Zheng JW, Zhang J, Hang BJ, He J, Li SP (2011) Characteristics of a 3- phenoxybenzoic acid degrading-bacterium and the construction of a engineering bacterium. Environ Sci 32:240–246Google Scholar
  14. Ermakova IT, Kiseleva NI, Shushkova T, Zharikov M, Zharikov GA, Leontievsky AA (2010) Bioremediation of glyphosate-contaminated soils. Appl Microbiol Biotechnol 88:585–594CrossRefGoogle Scholar
  15. Fenlon AK, Andreou K, Jones CK, Semple TK (2011a) The extractability and mineralisation of cypermethrin aged in four UK soils. Chemosphere 82:187–192CrossRefGoogle Scholar
  16. Fenlon AK, Jones CK, Semple TK (2011b) The effect of soil:water ratios on the induction of isoproturon, cypermethrin and diazinon mineralisation. Chemosphere 82:163–168CrossRefGoogle Scholar
  17. Gan J, Spurlock F, Hendley P, Weston D (2008) Synthetic pyrethroids: occurrence and effects in aquatic environments. American Chemical Society, WashingtonCrossRefGoogle Scholar
  18. Gu XZ, Zhang GY, Chen L, Dai RL, Yu YC (2008) Persistence and dissipation of synthetic pyrethroid pesticides in red soils from the Yangtze River Delta area. Environ Geochem Health 30:67–77CrossRefGoogle Scholar
  19. Guo P, Wang BZ, Hang BJ, Li L, Ali SW, He J, Li SP (2009) Pyrethroid-degrading Sphingobium sp. JZ-2 and the purification and characterization of a novel pyrethroid hydrolase. Int Biodeterior Biodegrad 63:1107–1112CrossRefGoogle Scholar
  20. Halden RU, Tepp SM, Halden BG, Dwyer DF (1999) Degradation of 3-phenoxybenzoic acid in soil by Pseudomonas pseudoalcaligenes POB310 (pPOB) and two modified Pseudomonas strains. Appl Environ Microbiol 65:3354–3359Google Scholar
  21. Han Y, Xia YK, Han JY, Zhou JP, Wang SL, Zhu PF, Zhao RC, Jin NZ, Song L, Wang XR (2008) The relationship of 3-PBA pyrethroids metabolite and male reproductive hormones among non-occupational exposure males. Chemosphere 72:785–790CrossRefGoogle Scholar
  22. Harms H, Schlosser D, Wick LY (2011) Untapped potential: exploiting fungi in bioremediation of hazardous chemicals. Nat Rev Microbiol 9:177–192CrossRefGoogle Scholar
  23. Hintzen EP, Lydy MJ, Belden JB (2009) Occurrence and potential toxicity of pyrethroids and other insecticides in bed sediments of urban streams in central Texas. Environ Pollut 157:110–116CrossRefGoogle Scholar
  24. Hong YF, Zhou J, Hong Q, Wang Q, Jiang JD, Li SP (2010) Characterization of a fenpropathrin-degrading strain and construction of a genetically engineered microorganism for simultaneous degradation of methyl parathion and fenpropathrin. J Environ Manage 91:2295–2300CrossRefGoogle Scholar
  25. Jin YX, Wang LG, Ruan M, Liu JW, Yang YF, Zhou C, Xu B, Fu ZW (2011a) Cypermethrin exposure during puberty induces oxidative stress and endocrine disruption in male mice. Chemosphere 84:124–130CrossRefGoogle Scholar
  26. Jin YX, Zheng SS, Fu ZW (2011b) Embryonic exposure to cypermethrin induces apoptosis and immunotoxicity in zebrafish (Danio rerio). Fish Shellfish Immunol 30:1049–1054CrossRefGoogle Scholar
  27. Jin YX, Zheng SS, Pu Y, Shu LJ, Sun LW, Liu WP, Fu ZW (2011c) Cypermethrin has the potential to induce hepatic oxidative stress, DNA damage and apoptosis in adult zebrafish (Danio rerio). Chemosphere 82:398–404CrossRefGoogle Scholar
  28. Katsuda Y (1999) Development of and future prospects for pyrethroid chemistry. Pestic Sci 55:775–782CrossRefGoogle Scholar
  29. Laffin B, Chavez M, Pine M (2010) The pyrethroid metabolites 3-phenoxybenzoic acid and 3-phenoxybenzyl alcohol do not exhibit estrogenic activity in the MCF-7 human breast carcinoma cell line or Sprague–Dawley rats. Toxicology 267:39–44CrossRefGoogle Scholar
  30. McKinlay R, Plant JA, Bell JN, Voulvoulis N (2008) Endocrine disrupting pesticides: implications for risk assessment. Environ Int 34:168–183CrossRefGoogle Scholar
  31. Meeker JD, Barr DB, Hauser R (2009) Pyrethroid insecticide metabolites are associated with sperm hormone levels in adult men. Reprod Toxicol 27: 155–160.CrossRefGoogle Scholar
  32. Mrozik A, Cycoń M, Piotrowska-Seget Z (2010) Changes of FAME profiles as a marker of phenol degradation in different soils inoculated with Pseudomonas sp. CF600. Int Biodeterior Biodegrad 64:86–96CrossRefGoogle Scholar
  33. Purnomo SA, Mori T, Takagi K, Kondo R (2011) Bioremediation of DDT contaminated soil using brown-rot fungi. Int Biodeterior Biodegrad 65:691–695CrossRefGoogle Scholar
  34. Sannino A, Bandini M, Bolzoni L (2003) Determination of pyrethroid pesticide residues in processed fruits and vegetables by gas chromatography with electron capture and mass spectrometric detection. J AOAC Int 86:101–108Google Scholar
  35. Shafer TJ, Meyer DA, Crofton KM (2005) Developmental neurotoxicity of pyrethroid insecticides: critical review and future research needs. Environ Health Persp 113:123–136CrossRefGoogle Scholar
  36. Shukla Y, Yadav A, Arora A (2002) Carcinogenic and cocarcinogenic potential of cypermethrin on mouse skin. Cancer Lett 182:33–41CrossRefGoogle Scholar
  37. Singh BK (2009) Organophosphorus-degrading bacteria: ecology and industrial applications. Nature Rev Microbiol 7:156–163CrossRefGoogle Scholar
  38. Singh BK, Walker A, Morgan JA, Wright DJ (2004) Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Appl Environ Microbiol 70:4855–4863CrossRefGoogle Scholar
  39. Singh BK, Walker A, Denis J, Wight DJ (2006) Bioremedial potential of fenamiphos and chlorpyrifos degrading isolates: influence of different environmental conditions. Soil Biol Biochem 38:2682–2693CrossRefGoogle Scholar
  40. Sun H, Xu XL, Xu LC, Song L, Hong X, Chen JF, Cui LB, Wang XR (2006) Antiandrogenic activity of pyrethroid pesticides and their metabolite in reporter gene assay. Chemosphere 66:474–479CrossRefGoogle Scholar
  41. Tallur PN, Megadi VB, Ninnekar HZ (2008) Biodegradation of cypermethrin by Micrococcus sp. strain CPN 1. Biodegradation 28:77–82CrossRefGoogle Scholar
  42. Tyler CR, Beresford N, van der Woning M, Sumpter JP, Thorpe K (2000) Metabolism and environmental degradation of pyrethroid insecticides produce compounds with endocrine activities. Environ Toxicol Chem 19:801–809CrossRefGoogle Scholar
  43. Wang XZ, Liu SS, Sun Y, Wu JY, Zhou YL, Zhang JH (2009a) Beta-cypermethrin impairs reproductive function in male mice by inducing oxidative stress. Theriogenology 72:599–611CrossRefGoogle Scholar
  44. Wang BZ, Guo P, Hang BJ, Li L, He J, Li SP (2009b) Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from Sphingobium sp. strain JZ-1 and characterization of the gene product. Appl Environ Microbiol 75:5496–5500CrossRefGoogle Scholar
  45. Weston DP, Lydy MJ (2010) Urban and agricultural sources of pyrethroid insecticides to the Sacramento-San Joaquin Delta of California. Environ Sci Technol 44:1833–1840CrossRefGoogle Scholar
  46. Weston DP, Holmes RW, Lydy MJ (2009) Residential runoff as a source of pyrethroid pesticides to urban creeks. Environ Pollut 157:287–294CrossRefGoogle Scholar
  47. Wolansky MJ, Harrill JA (2008) Neurobehavioral toxicology of pyrethroid insecticides in adult animals: a critical review. Neurotoxicol Teratol 30:55–78CrossRefGoogle Scholar
  48. Xie WJ, Zhou JM, Wang HY, Chen XQ (2008) Effect of nitrogen on the degradation of cypermethrin and its metabolite 3-phenoxybenzoic acid in soil. Pedosphere 18:638–644CrossRefGoogle Scholar
  49. Xu YX, Sun JQ, Li XH, Li SP, Chen Y (2007) Study on cooperating degradation of cypermethrin and 3-phenoxybenzoic acid by two bacteria strains. Acta Microbiol Sin 47:834–837Google Scholar
  50. Xu GM, Zheng W, Li YY, Wang SH, Zhang JS, Yan YC (2008) Biodegradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol by a newly ioslated Paracoccus sp. TRP. Int Biodeterior Biodegrad 62:51–56CrossRefGoogle Scholar
  51. Yuan C, Wang C, Gao SQ, Kong TT, Chen L, XF L, Song L, Wang YB (2010) Effects of permethrin, cypermethrin and 3-phenoxybenzoic acid on rat sperm motility in vitro evaluated with computer-assisted sperm analysis. Toxicol in Vitro 24:382–386CrossRefGoogle Scholar
  52. Zhang L, Gao X, Liang P (2007) Beta-cypermethrin resistance associated with high carboxylesterase activities in a strain of house fly, Musca domestica (Diptera: Muscidae). Pestic Biochem Physiol 89:65–72CrossRefGoogle Scholar
  53. Zhang C, Jia L, Wang SH, Qu J, Xu LL, Shi HH, Yan YC (2010) Biodegradation of beta-cypermethrin by two Serratia spp. with different cell surface hydrophobicity. Bioresource Technol 101:3423–3429CrossRefGoogle Scholar
  54. Zhang C, Wang SH, Yan YC (2011) Isomerization and biodegradation of beta-cypermethrin by Pseudomonas aeruginosa CH7 with biosurfactant production. Bioresource Technol 102:7139–7146CrossRefGoogle Scholar
  55. Zhuang RS, Chen HL, Yao J, Li Z, Burnet EJ, Choi MFM (2011) Impact of beta-cypermethrin on soil microbial community associated with its bioavailability: a combined study by isothermal microcalorimetry and enzyme assay techniques. J Hazard Mater 189:323–328CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Shaohua Chen
    • 1
  • Peng Geng
    • 1
  • Ying Xiao
    • 1
  • Meiying Hu
    • 1
    Email author
  1. 1.Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Laboratory of Insect ToxicologySouth China Agricultural UniversityGuangzhouPeople’s Republic of China

Personalised recommendations