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Applied Microbiology and Biotechnology

, Volume 93, Issue 2, pp 859–869 | Cite as

Anaerobic digestibility of marine microalgae Phaeodactylum tricornutum in a lab-scale anaerobic membrane bioreactor

  • Carlos Zamalloa
  • Jo De Vrieze
  • Nico Boon
  • Willy VerstraeteEmail author
Bioenergy and biofuels

Abstract

The biomass of industrially grown Phaeodactylum tricornutum was subjected in a novel way to bio-methanation at 33°C, i.e., in an anaerobic membrane bioreactor (AnMBR) at a hydraulic retention time of 2.5 days, at solid retention times of 20 to 10 days and at loading rates in the range of 2.6–5.9 g biomass-COD L−1 day−1 with membrane fluxes ranging from 1 to 0.8 L m−2 h−1. The total COD recovered as biogas was in the order of 52%. The input suspension was converted to a clear effluent rich in total ammonium nitrogen (546 mg TAN L−1) and phosphate (141 mg PO4-P L−1) usable as liquid fertilizer. The microbial community richness, dynamics, and organization in the reactor were interpreted using the microbial resource management approach. The AnMBR communities were found to be moderate in species richness and low in dynamics and community organization relative to UASB and conventional CSTR sludges. Quantitative polymerase chain reaction analysis revealed that Methanosaeta sp. was the dominant acetoclastic methanogen species followed by Methanosarcina sp. This work demonstrated that the use of AnMBR for the digestion of algal biomass is possible. The fact that some 50% of the organic matter is not liquefied means that the algal particulates in the digestate constitute a considerable fraction which should be valorized properly, for instance as slow release organic fertilizer. Overall, 1 kg of algae dry matter (DM) could be valorized in the form of biogas (€2.07), N and P in the effluent (€0.02) and N and P in the digestate (€0.04), thus totaling about €2.13 per kilogram algae DM.

Keywords

Algae biomass Mesophilic Green energy Biogas Methane Microbial resource management 

Notes

Acknowledgments

This work was supported by the Institute for the Promotion of Innovation by Science and Technology-Strategic Basic Research (IWT-SBO) Sunlight Project-Lipid-based, high value products and renewable energy from microalgae grant 80031 and Ghent University grant 179I16D9W. We thank Jessica Benner and Tom Hennebel for critically reading the manuscript and Tim Lacoere for his valuable help in the molecular analysis.

Supplementary material

253_2011_3624_MOESM1_ESM.docx (1.3 mb)
ESM 1 (DOCX 1,343 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Carlos Zamalloa
    • 1
  • Jo De Vrieze
    • 1
  • Nico Boon
    • 1
  • Willy Verstraete
    • 1
    Email author
  1. 1.Faculty of Bioscience Engineering; Laboratory of Microbial Ecology and Technology (LabMET)Ghent UniversityGhentBelgium

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