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Applied Microbiology and Biotechnology

, Volume 93, Issue 5, pp 2147–2159 | Cite as

Characterization and regulation of the 2,3-butanediol pathway in Serratia marcescens

  • Ben Rao
  • Liao Yuan Zhang
  • Jian’an Sun
  • Gang Su
  • Dongzhi Wei
  • Ju Chu
  • Jiawen Zhu
  • Yaling ShenEmail author
Applied microbial and cell physiology

Abstract

Serratia marcescens has been proved to be a potential strain for industrial 2,3-butanediol production for its high yield, productivity, and other advantages. In this study, the genes slaA, slaB, slaC, and slaR were successfully cloned which were further confirmed to be encoding acetolactate decarboxylase, acetolactate synthase, 2,3-butanediol dehydrogenase, and a LysR-like regulator, respectively. Unlike in Klebsiella sp. or Klebsiella pneumonie and Vibrio sp. or Vibrio cholerae, the gene slaC is separated from other genes. Then it showed that two regulators, SwrR and SlaR, are in charge of this process by exerting effect on the transcription of genes slaA and slaB. By contrast, the expression of gene slaC is unaffected by the two regulators. It means that these two regulators affect the production of 2,3-butanediol by regulating the production of acetoin. Based on these findings, we successfully accelerated the 2,3-butanediol production by inactivation of gene swrR. The obtained results and further investigations should lead to a more suitable fermentation strategy and strain improvement which would be applicable to the industrial production of 2,3-butanediol.

Keywords

Serratia marcescens Acetoin 2,3-Butanediol Regulation 

Notes

Acknowledgments

We thank Staffan Kjelleberg (The University of New South Wales) for providing S. marcescens MG1. This work was supported by Chinese National Program for High Technology and Development (863 program; no. 2006AA02Z243), Shanghai Leading Academic Discipline Project (project B505) and National Special Fund for State Key Laboratory of Bioreactor Engineering (no. 2060204).

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Ben Rao
    • 1
  • Liao Yuan Zhang
    • 2
  • Jian’an Sun
    • 1
  • Gang Su
    • 1
  • Dongzhi Wei
    • 1
  • Ju Chu
    • 3
  • Jiawen Zhu
    • 4
  • Yaling Shen
    • 1
    Email author
  1. 1.State Key Laboratory of Bioreactor Engineering, New World Institute of BiotechnologyEast China University of Science and TechnologyShanghaiPeople’s Republic of China
  2. 2.School of Life ScienceFujian Agriculture and Forestry UniversityFuzhouPeople’s Republic of China
  3. 3.State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiPeople’s Republic of China
  4. 4.Chemical Engineering Research CenterEast China University of Science and TechnologyShanghaiPeople’s Republic of China

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