Applied Microbiology and Biotechnology

, Volume 93, Issue 3, pp 1057–1063 | Cite as

High-level production of 1,3-propanediol from crude glycerol by Clostridium butyricum AKR102a

  • Erik Wilkens
  • Anne Katrin Ringel
  • Diana Hortig
  • Thomas Willke
  • Klaus-Dieter Vorlop
Biotechnological products and process engineering

Abstract

The aim of this study was to optimize a biotechnological process for the production of 1,3-propanediol (1,3-PD) based on low-quality crude glycerol derived from biodiesel production. Clostridium butyricum AKR102a was used in fed-batch fermentations in 1-L and 200-L scale. The newly discovered strain is characterized by rapid growth, high product tolerance, and the ability to use crude glycerol at the lowest purity directly gained from a biodiesel plant side stream. Using pure glycerol, the strain AKR102 reached 93.7 g/L 1,3-PD with an overall productivity of 3.3 g/(L*h). With crude glycerol under the same conditions, 76.2 g/L 1,3-PD was produced with a productivity of 2.3 g/(L*h). These are among the best results published so far for natural producers. The scale up to 200 L was possible. Due to the simpler process design, only 61.5 g/L 1,3-PD could be reached with a productivity of 2.1 g/(L*h).

Keywords

Fermentation Crude glycerol 1,3-Propanediol Clostridium butyricum 

Notes

Acknowledgments

This project was funded by the German Federal Ministry of Education and Research (BMBF) (IG-Biotech 0315026E). I want to thank PD Dr. Udo Rau and Wolfgang Graßl from the Technische Universität Braunschweig for the kind technical provision of the large-scale fermenter and the assistance during the 200-L fermentation.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Erik Wilkens
    • 1
  • Anne Katrin Ringel
    • 1
  • Diana Hortig
    • 1
  • Thomas Willke
    • 1
  • Klaus-Dieter Vorlop
    • 1
  1. 1.Johann Heinrich von Thünen-Institut (vTI)Institute of Agricultural Technology and Biosystems Engineering (AB)BraunschweigGermany

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