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Applied Microbiology and Biotechnology

, Volume 93, Issue 1, pp 261–272 | Cite as

Sequencing and analysis of three plasmids from Lactobacillus casei TISTR1341 and development of plasmid-derived Escherichia coli–L. casei shuttle vectors

  • Marutpong Panya
  • Viraphong Lulitanond
  • Sithichoke Tangphatsornruang
  • Wises Namwat
  • Rungnapha Wannasutta
  • Namfon Suebwongsa
  • Baltasar MayoEmail author
Applied genetics and molecular biotechnology

Abstract

Pyrosequencing followed by conventional PCR and sequencing was used to determine the complete nucleotide sequence of three plasmids (pRCEID2.9, pRCEID3.2, and pRCEID13.9) from the Lactobacillus casei strain TISTR1341. The plasmid sequences were found to be almost identical, respectively, to those of pLA106, pLA105, and pLA103 from Lactobacillus acidophilus strain TK8912, suggesting that these strains may be related. Sequence analysis and comparison indicated that pRCEID2.9 replicates by a rolling circle (RC) mechanism, while pRCEID3.2 and pRCEID13.9 probably follow a theta-type mode of replication. Replicons of pRCEID2.9 and pRCEID13.9 were used to develop Escherichia coli/L. casei compatible shuttle vectors, which were stably maintained in different genetic backgrounds. Real-time quantitative PCR analysis showed copy numbers of around 4 and 15, respectively, for the pRCEID13.9- and pRCEID2.9-derived shuttle vectors per chromosome equivalent. The functionality of vector pRCEID-LC13.9 was proved by cloning and expressing in L. casei of a green fluorescent protein gene variant from Aequorea victoria under the control of the promoter from a homologous lactate dehydrogenase gene. The new vectors might complement those currently in use for the exploitation of L. casei as a cellular factory and in other biotechnological applications.

Keywords

Lactobacillus casei Plasmid Cloning vectors Lactic acid bacteria Probiotics 

Notes

Acknowledgments

This study was supported by grants from the Office of the Higher Education Commission through CHE Ph.D. Scholarship (to M.P.) and through the Higher Education Research Promotion and National Research University Project (to V.L.), the National Center for Genetics Engineering and Biotechnology (BIOTEC), and the Research and Diagnostic Center for Emerging Infectious Diseases (RCEID), Khon Kaen University, Thailand. Further support was obtained through a project from the Spanish Ministry of Science and Innovation (ref. AGL2007-61869-ALI) (to B.M.).

References

  1. Acedo-Félix E, Pérez-Martínez G (2003) Significant differences between Lactobacillus casei subsp. casei ATCC 393T and a commonly used plasmid-cured derivative revealed by a polyphasic study. Int J Syst Evol Microbiol 53:67–75CrossRefGoogle Scholar
  2. An H-Y, Miyamoto T (2006) Cloning and sequencing of plasmid pLC494 from human intestinal Lactobacillus casei: construction of an Escherichia coliLactobacillus shuttle vector. Plasmid 55:128–134CrossRefGoogle Scholar
  3. Benachour A, Frère J, Flahaut S, Novel G, Auffray Y (1997) Molecular analysis of the replication region of the theta-replicating plasmid pUCL287 from Tetragenococcus (Pediococcus) halophilus ATCC33315. Mol Gen Genet 255:504–513CrossRefGoogle Scholar
  4. Chassy BM, Alpert C-A (1989) Molecular characterization of the plasmid-encoded lactose-PTS of Lactobacillus casei. FEMS Microbiol Rev 63:157–166CrossRefGoogle Scholar
  5. Chassy BM, Flickinger JL (1987) Transformation of Lactobacillus casei by electroporation. FEMS Microbiol Lett 44:173–177CrossRefGoogle Scholar
  6. del Solar G, Giraldo R, Ruiz-Echevarría MJ, Espinosa M, Díaz-Orejas R (1998) Replication and control of circular bacterial plasmids. Microbiol Mol Biol Rev 62:434–464Google Scholar
  7. Desmond C, Ross PR, Fitzgerald G, Stanton C (2005) Sequence analysis of the plasmid genome of the probiotic strain Lactobacillus paracasei NFBC338 which includes the plasmids pCD01 and pCD02. Plasmid 54:160–175CrossRefGoogle Scholar
  8. Dower WJ, Miller JF, Ragsdale CW (1988) High efficiency of transformation of Escherichia coli by high voltage electroporation. Nuc Acids Res 16:6127–6145CrossRefGoogle Scholar
  9. Felis GE, Dellaglio F (2007) Taxonomy of lactobacilli and bifidobacteria. Curr Issues Intest Microbiol 8:44–61Google Scholar
  10. Flórez AB, Ammor MS, Álvarez-Martín P, Margolles A, Mayo B (2006) Molecular analysis of tet(W) gene-mediated tetracycline resistance in dominant intestinal Bifidobacterium species from healthy humans. Appl Environ Microbiol 72:7377–7379CrossRefGoogle Scholar
  11. García P, Ladero V, Suárez JE (2003) Analysis of the morphogenetic cluster and genome of the temperate Lactobacillus casei bacteriophage A2. Arch Virol 148:1051–1070CrossRefGoogle Scholar
  12. Gasson MJ (1983) Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J Bacteriol 154:1–9Google Scholar
  13. Hammes WP, Hertel C (2006) The genera Lactobacillus and Carnobacterium. In: Dworking M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds) The Prokaryotes, Part I, Section 1.2. Springer, New York, pp 4320–4403Google Scholar
  14. Ho PS, Kwang J, Lee YK (2005) Intragastric administration of Lactobacillus casei expressing transmissible gastroentritis coronavirus spike glycoprotein induced specific antibody production. Vaccine 23:1335–1342CrossRefGoogle Scholar
  15. Kanatani K, Tahara T, Oshimura M, Sano K, Umezawa C (1995a) Identification of the replication region of Lactobacillus acidophilus plasmid pLA103. FEMS Microbiol Lett 133:127–130CrossRefGoogle Scholar
  16. Kanatani K, Tahara T, Oshimura M, Sano K, Umezawa C (1995b) Characterization of a small cryptic plasmid, pLA105, from Lactobacillus acidophilus TK8912. J Ferment Bioengin 80:394–399CrossRefGoogle Scholar
  17. Kanatani K, Oshimura M, Sano K (1995c) Isolation and characterization of acidocin A and cloning of the bacteriocin gene from Lactobacillus acidophilus. Appl Environ Microbiol 61:1061–1067Google Scholar
  18. Kankainen M, Paulin L, Tynkkynen S, von Ossowski I, Reunanen J, Partanen P, Satokari R, Vesterlund S, Hendrickx AP, Lebeer S, De Keersmaecker SC, Vanderleyden J, Hämäläinen T, Laukkanen S, Salovuori N, Ritari J, Alatalo E, Korpela R, Mattila-Sandholm T, Lassig A, Hatakka K, Kinnunen KT, Karjalainen H, Saxelin M, Laakso K, Surakka A, Palva A, Salusjärvi T, Auvinen P, de Vos WM (2009) Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human-mucus binding protein. Proc Natl Acad Sci USA 106:17193–17198CrossRefGoogle Scholar
  19. Kiewiet R, Bron S, de Jonge K, Venema G, Seegers JFML (1993) Theta replication of the lactococcal plasmid pWV02. Mol Microbiol 10:319–327CrossRefGoogle Scholar
  20. Kim YG, Ohta T, Takahashi T, Kushiro A, Nomoto K, Yokokura T, Okada N, Danbara H (2006) Probiotic Lactobacillus casei activates innate immunity via NF-kappaB and p38 MAP kinase signaling pathways. Microb Infect 8:994–1005CrossRefGoogle Scholar
  21. Kojic M, Lozo J, Jovcic B, Strahinic I, Fira D, Topisirovic L (2010) Construction of a new shuttle vector and its use for cloning and expression of two plasmid-encoded bacteriocins from Lactobacillus paracasei subsp. paracasei BGSJ2-8. Int J Food Microbiol 140:117–124CrossRefGoogle Scholar
  22. Lee JS, Poo H, Han DP, Hong SP, Kim K, Cho MW, Kim E, Sung MH, Kim CJ (2006a) Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice. J Virol 80:4079–4087CrossRefGoogle Scholar
  23. Lee C, Kim J, Shin SG, Hwang S (2006b) Absolute and relative Q-PCR quantification of plasmid copy number in Escherichia coli. J Biotechnol 123:273–280CrossRefGoogle Scholar
  24. Leenhouts KJ, Tolner B, Bron S, Kok J, Venema G, Seegers JF (1991) Nucleotide sequence and characterization of the broad-host-range lactococcal plasmid pWVO1. Plasmid 26:55–66CrossRefGoogle Scholar
  25. Maassen CB (1999) A rapid and safe plasmid isolation method for efficient engineering of recombinant lactobacilli expressing immunogenic or tolerogenic epitopes for oral administration. J Immunol Methods 223:131–136CrossRefGoogle Scholar
  26. Mazé A, Boël G, Zúñiga M, Bourand A, Loux V, Yebra MJ, Monedero V, Correia K, Jacques N, Beaufils S, Poncet S, Joyet P, Milohanic E, Casarégola S, Auffray Y, Pérez-Martínez G, Gibrat JF, Zagorec M, Francke C, Hartke A, Deutscher J (2010) Complete genome sequence of the probiotic Lactobacillus casei strain BL23. J Bacteriol 192:2647–2648CrossRefGoogle Scholar
  27. O’sullivan DJ, Klaenhammer TR (1993) Rapid Mini-Prep isolation of high-quality plasmid DNA from Lactococcus and Lactobacillus spp. Appl Environ Microbiol 59:2730–2733Google Scholar
  28. Ruiz-Masó JA, Lurz R, Espinosa M, del Solar G (2007) Interactions between the RepB initiator protein of plasmid pMV158 and two distant DNA regions within the origin of replication. Nuc Acids Res 35:1230–1244CrossRefGoogle Scholar
  29. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
  30. Sano K, Otani M, Okada Y, Kawamura R, Umesaki M, Ohi Y, Umezawa C, Kanatani K (1997) Identification of the replication region of the Lactobacillus acidophilus plasmid pLA106. FEMS Microbiol Lett 148:223–226CrossRefGoogle Scholar
  31. Shareck J, Choi Y, Lee B, Míguez CB (2004) Cloning vectors based on cryptic plasmids isolated from lactic acid bacteria: their characteristics and potential applications in biotechnology. Crit Rev Biotechnol 24:155–208CrossRefGoogle Scholar
  32. Shimizu-Kadota M (1987) Properties of lactose plasmid pLY101 in Lactobacillus casei. Appl Environ Microbiol 53:2987–2991Google Scholar
  33. Sudhamani M, Ismaiel E, Geis A, Batish V, Heller KJ (2008) Characterisation of pSMA23, a 3.5 kbp plasmid of Lactobacillus casei, and application for heterologous expression in Lactobacillus. Plasmid 59:11–19CrossRefGoogle Scholar
  34. Thanaruttikannont T (1996) Use of lactic acid bacteria as probiotic supplement in chicken feed. MS thesis, Chulalongkorn University, Chulalongkorn, ThailandGoogle Scholar
  35. Wang TT, Lee BH (1997) Plasmids in Lactobacillus. Crit Rev Biotechnol 17:227–272CrossRefGoogle Scholar
  36. Yao XY, Wang HM, Li DJ, Yuan MM, Wang XL, Yu M, Wang MY, Zhu Y, Meng Y (2004) Inoculation of Lactobacillus expressing hCG beta in the vagina induces an anti-hCG beta antibody response in murine vaginal mucosa. J Reproduct Immunol 63:111–122CrossRefGoogle Scholar
  37. Zhang W, Yu D, Sun Z, Wu R, Chen X, Bao Q, Meng H, Hu S, Zhang H (2008) Complete nucleotide sequence of plasmid plca36 isolated from Lactobacillus casei Zhang. Plasmid 60:131–135CrossRefGoogle Scholar
  38. Zhang H, Hao Y, Zhang D, Luo Y (2010a) Characterization of the cryptic plasmid pTXW from Lactobacillus paracasei TXW. Plasmid 65:1–7CrossRefGoogle Scholar
  39. Zhang W, Yu D, Sun Z, Wu R, Chen X, Chen W, Meng H, Hu S, Zhang H (2010b) Complete genome sequence of Lactobacillus casei Zhang, a new probiotic strain isolated from traditional homemade koumiss in Inner Mongolia, China. J Bacteriol 192:5268–5269CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Marutpong Panya
    • 1
    • 2
  • Viraphong Lulitanond
    • 1
  • Sithichoke Tangphatsornruang
    • 3
  • Wises Namwat
    • 1
  • Rungnapha Wannasutta
    • 1
  • Namfon Suebwongsa
    • 1
  • Baltasar Mayo
    • 2
    Email author
  1. 1.Research and Diagnostic Center for Emerging Infectious Diseases (RCEID), Faculty of MedicineKhon Kaen UniversityKhon KaenThailand
  2. 2.Departamento de Microbiología y BioquímicaInstituto de Productos Lácteos de Asturias (CSIC)VillaviciosaSpain
  3. 3.National Center for Genetic Engineering and BiotechnologyNational Science Technology Development AgencyKlong LuangThailand

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