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Applied Microbiology and Biotechnology

, Volume 91, Issue 6, pp 1553–1559 | Cite as

Co-fermentation of cellulose/xylan using engineered industrial yeast strain OC-2 displaying both β-glucosidase and β-xylosidase

  • Satoshi Saitoh
  • Tsutomu Tanaka
  • Akihiko Kondo
Biotechnological Products and Process Engineering

Abstract

We constructed a recombinant industrial Saccharomyces cerevisiae yeast strain OC2-AXYL2-ABGL2-Xyl2 by inserting two copies of the β-glucosidase (BGL) and β-xylosidase (XYL) genes, and a gene cassette for xylose assimilation in the genome of yeast strain OC-2HUT. Both BGL and XYL were expressed on the yeast cell surface with high enzyme activities. Using OC2-AXYL2-ABGL2-Xyl2, we performed ethanol fermentation from a mixture of powdered cellulose (KC-flock) and Birchwood xylan, with the additional supplementation of a 30-g/l Trichoderma reesei cellulase complex mixture. The ethanol yield (gram per gram of added cellulases) of the strain OC2-AXYL2-ABGL2-Xyl2 increased approximately 2.5-fold compared to that of strain OC2-Xyl2, which lacked β-glucosidase and β-xylosidase activities. Notably, the concentration of additional T. reesei cellulase was reduced from 30 to 24 g/l without affecting ethanol production. The BGL- and XYL-displaying industrial yeast of the strain OC2-AXYL2-ABGL2-Xyl2 represents a promising yeast for reducing cellulase consumption of ethanol fermentation from lignocellulosic biomass by compensating for the inherent weak BGL and XYL activities of T. reesei cellulase complexes.

Keywords

Saccharomyces cerevisiae β-Xylosidase β-Glucosidase Xylose fermentation Cellulosic ethanol 

Notes

Acknowledgments

This work was partially supported by Special Coordination Funds for Promoting Science and Technology under the project of Creation of Innovation Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction Kobe), MEXT, Japan.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Toyota Biotechnology & Afforestation LaboratoryToyota Motor CoMiyoshiJapan
  2. 2.Organization of Advanced Science and TechnologyKobe UniversityKobeJapan
  3. 3.Department of Chemical Science and Engineering, Graduate School of EngineeringKobe UniversityKobeJapan

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