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Applied Microbiology and Biotechnology

, Volume 92, Issue 3, pp 597–607 | Cite as

Reductive dehalogenation mediated initiation of aerobic degradation of 2-chloro-4-nitrophenol (2C4NP) by Burkholderia sp. strain SJ98

  • Janmejay Pandey
  • Hermann J. Heipieper
  • Archana Chauhan
  • Pankaj Kumar Arora
  • Dhan Prakash
  • M. Takeo
  • Rakesh K. JainEmail author
Applied Microbial and Cell Physiology

Abstract

Burkholderia sp. strain SJ98 (DSM 23195) was previously isolated and characterized for degradation and co-metabolic transformation of a number nitroaromatic compounds. In the present study, we evaluated its metabolic activity on chlorinated nitroaromatic compounds (CNACs). Results obtained during this study revealed that strain SJ98 can degrade 2-chloro-4-nitrophenol (2C4NP) and utilize it as sole source of carbon, nitrogen, and energy under aerobic conditions. The cells of strain SJ98 removed 2C4NP from the growth medium with sequential release of nearly stoichiometric amounts of chloride and nitrite in culture supernatant. Under aerobic degradation conditions, 2C4NP was transformed into the first intermediate that was identified as p-nitrophenol by high-performance liquid chromatography, LCMS-TOF, and GC-MS analyses. This transformation clearly establishes that the degradation of 2C4NP by strain SJ98 is initiated by “reductive dehalogenation”; an initiation mechanism that has not been previously reported for microbial degradation of CNAC under aerobic conditions.

Keywords

Chlorinated nitroaromatic compounds Burkholderia Aerobic degradation 

Notes

Acknowledgments

This study was supported in part by the Department of Biotechnology (DBT), India and the Council for Scientific and Industrial Research (CSIR), India. We are thankful to Ms. Mahima Pant and Mr. Vivek Mahajan for their technical help during this study. JP, AC, and PKA acknowledge the research scholarship provided by CSIR, India.

Supplementary material

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Janmejay Pandey
    • 1
  • Hermann J. Heipieper
    • 2
  • Archana Chauhan
    • 1
  • Pankaj Kumar Arora
    • 1
  • Dhan Prakash
    • 1
  • M. Takeo
    • 3
  • Rakesh K. Jain
    • 1
    Email author
  1. 1.Institute of Microbial Technology (CSIR)ChandigarhIndia
  2. 2.Department of Environmental BiotechnologyHelmholtz Centre for Environmental Research—UFZLeipzigGermany
  3. 3.Division of Material Engineering, Graduate Institute of EngineeringHimeji Institute of TechnologyHyogoJapan

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