Applied Microbiology and Biotechnology

, Volume 91, Issue 3, pp 713–730 | Cite as

Identifying genes that impact on aroma profiles produced by Saccharomyces cerevisiae and the production of higher alcohols

Applied Genetics and Molecular Biotechnology

Abstract

During alcoholic fermentation, many volatile aroma compounds are formed by Saccharomyces cerevisiae, including esters, fatty acids, and higher alcohols. While the metabolic network that leads to the formation of these compounds is reasonably well mapped, surprisingly little is known about specific enzymes involved in specific reactions, the regulation of the network, and the physiological roles of individual pathways within the network. Furthermore, different yeast strains tend to produce significantly different aroma profiles. These differences are of tremendous biotechnological interest, since producers of alcoholic beverages such as wine and beer are searching for means to diversify and improve their product range. Various factors such as the redox, energy, and nutritional balance of a cell have previously been suggested to directly or indirectly affect and regulate the network. To gain a better understanding of the regulations and physiological role of this network, we screened a subset of the EUROSCARF strain deletion library for genes that, when deleted, would impact most significantly on the aroma profile produced under fermentative conditions. The 10 genes whose deletion impacted most significantly on higher alcohol production were selected and further characterized to assess their mode of action within or on this metabolic network. This is the first description of a large-scale screening approach using aroma production as the primary selection criteria, and the data suggest that many of the identified genes indeed play central and direct roles within the aroma production network of S. cerevisiae.

Keywords

Ehrlich pathway Branched chain amino acid metabolism Higher alcohols Wine aroma 

Supplementary material

253_2011_3237_MOESM1_ESM.tif (3.7 mb)
High resolution image (TIFF 3784 kb)
253_2011_3237_MOESM2_ESM.txt (18 kb)
Table A1Results of t test of all strains that were screened initially (TXT 17 kb)
253_2011_3237_MOESM3_ESM.doc (26 kb)
ESM 1(DOC 26 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Gustav Styger
    • 1
  • Dan Jacobson
    • 1
  • Florian F. Bauer
    • 1
  1. 1.Institute for Wine BiotechnologyStellenbosch UniversityStellenboschSouth Africa

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