Applied Microbiology and Biotechnology

, Volume 91, Issue 6, pp 1571–1579 | Cite as

Production, purification, and characterization of a novel killer toxin from Kluyveromyces siamensis against a pathogenic yeast in crab

  • Muhammad Aslam Buzdar
  • Zhe Chi
  • Qi Wang
  • Ming-Xia Hua
  • Zhen-Ming ChiEmail author
Biotechnologically Relevant Enzymes and Proteins


The yeast Kluyveromyces siamensis HN12-1 isolated from mangrove ecosystem was found to be able to produce killer toxin against the pathogenic yeast (Metschnikowia bicuspidata WCY) in crab. When the killer yeast was grown in the medium with pH 4.0 and 0.5% NaCl and at 25 °C, it could produce the highest amount of killer toxin against the pathogenic yeast M. bicuspidata WCY. The killing activity of the purified killer toxin against the pathogenic yeast M. bicuspidata WCY was the highest when it was incubated at 25 °C in the assay medium without added NaCl and pH 4.0. The molecular weight of the purified killer toxin was 66.4 kDa. The killer toxin produced by the yeast strain HN12-1 could kill only the whole cells of M. bicuspidata WCY among all the yeast species tested in this study. This is the first time to report that the killer toxin produced by the yeast K. siamensis HN12-1 isolated from the mangrove ecosystem only killed pathogenic yeast M. bicuspidata WCY.


Killer toxin Killer yeast Mangrove ecosystem Pathogenic yeast Yeast relationship 



This work was supported by grant 30771645 from National Natural Science Foundation of China.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Muhammad Aslam Buzdar
    • 1
  • Zhe Chi
    • 1
  • Qi Wang
    • 1
  • Ming-Xia Hua
    • 1
  • Zhen-Ming Chi
    • 1
    Email author
  1. 1.UNESCO Chinese Centre of Marine BiotechnologyOcean University of ChinaQingdaoChina

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