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Characterization of a bifunctional xylanase/endoglucanase from yak rumen microorganisms

  • Lei Chang
  • Mozhu Ding
  • Lei Bao
  • Yingzhi Chen
  • Jungang ZhouEmail author
  • Hong LuEmail author
Biotechnologically Relevant Enzymes and Proteins

Abstract

A new gene, RuCelA, encoding a bifunctional xylanase/endoglucanase, was cloned from a metagenomic library of yak rumen microorganisms. RuCelA showed activity against xylan and carboxymethylcellulose (CMC), suggesting bifunctional xylanase/endoglucanase activity. The optimal conditions for xylanase and endoglucanase activities were 65°C, pH 7.0 and 50°C, pH 5.0, respectively. In addition, the presence of Co+ and Co2+ can greatly improve RuCelA's endoglucanase activity, while inhibits its xylanase activity. Further examination of substrate preference showed a higher activity against barley glucan and lichenin than against xylan and CMC. Using xylan and barley glucan as substrates, RuCelA displayed obvious synergistic effects with β-1,4-xylosidase and β-1,4-glucosidase. Generation of soluble oligosaccharides from lignocellulose is the key step in bioethanol production, and it is greatly notable that RuCelA can produce xylo-oligosaccharides and cello-oligosaccharides in the continuous saccharification of pretreated rice straw, which can be further degraded into fermentable sugars. Therefore, the bifunctional RuCelA distinguishes itself as an ideal candidate for industrial applications.

Keywords

Bifunctional Metagenomic Xylanase Endoglucanase 

Notes

Acknowledgments

This work was supported by Chinese High-tech Research and Development Program 2007AA021302.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.State Key Laboratory of Genetic EngineeringFudan UniversityShanghaiPeople’s Republic of China
  2. 2.School of PharmacyChina Pharmaceutical UniversityNanjingPeople’s Republic of China

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