Applied Microbiology and Biotechnology

, Volume 89, Issue 4, pp 1255–1264 | Cite as

Ethanol production from biodiesel-derived crude glycerol by newly isolated Kluyvera cryocrescens

  • Won Jae Choi
  • Maria Regina Hartono
  • Weng Heng Chan
  • Suan Siong Yeo
Bioenergy and Biofuels


The rapidly expanding market for biodiesel has increased the supply and reduced the cost of glycerol, making it an attractive sustainable feed stock for the fuel and chemical industry. Glycerol-based biorefinery is the microbial fermentation of crude glycerol to produce fuels and chemicals. A major challenge is to obtain microbes tolerant to inhibitors such as salts and organic solvents present in crude glycerol. Microbial screening was attempted to isolate novel strain capable of growing on crude glycerol as a sole carbon source. The newly isolated bacteria, identified as nonpathogenic Kluyvera cryocrescens S26 could convert biodiesel-derived crude glycerol to ethanol with high yield and productivity. The supplementation of nutrients such as yeast extract resulted in distinguished enhancement in cell growth as well as ethanol productivity under anaerobic condition. When glycerol fermentation is performed under microaerobic condition, there is also a remarkable improvement in cell growth, ethanol productivity and yield, compared with those under strict anaerobic condition. In batch fermentation under microaerobic condition, K. cryocrescens S26 produced 27 g/l of ethanol from crude glycerol with high molar yield of 80% and productivity of 0.61 g/l/h.


Crude glycerol Ethanol Fermentation Kluyvera cryocrescens 



This work was supported by Science and Engineering Research Council of Agency for Science, Technology and Research (A*STAR), Singapore, grant number ICES/07-173A02.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Won Jae Choi
    • 1
  • Maria Regina Hartono
    • 1
  • Weng Heng Chan
    • 1
  • Suan Siong Yeo
    • 1
  1. 1.Institute of Chemical and Engineering SciencesAgency for Science, Technology and Research (A*STAR)Jurong IslandSingapore

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