Applied Microbiology and Biotechnology

, Volume 90, Issue 1, pp 361–368

Frequent concomitant presence of Desulfitobacterium spp. and “Dehalococcoides” spp. in chloroethene-dechlorinating microbial communities

  • Katia Rouzeau-Szynalski
  • Julien Maillard
  • Christof Holliger
Environmental Biotechnology

DOI: 10.1007/s00253-010-3042-0

Cite this article as:
Rouzeau-Szynalski, K., Maillard, J. & Holliger, C. Appl Microbiol Biotechnol (2011) 90: 361. doi:10.1007/s00253-010-3042-0

Abstract

The presence of chloroethene dechlorination activity as well as several bacterial genera containing mainly organohalide-respiring members was investigated in 34 environmental samples from 18 different sites. Cultures inoculated with these environmental samples on tetrachloroethene and amended weekly with a seven organic electron donor mixture resulted in 11 enrichments with cis-DCE, ten with VC, and 11 with ethene as dechlorination end product, and only two where no dechlorination was observed. “Dehalococcoides” spp. and Desulfitobacterium spp. were detected in the majority of the environmental samples independently of the dechlorination end product formed. The concomitant presence of Dehalococcoides spp. and Desulfitobacterium spp. in the majority of the enrichments suggested that chloroethene dechlorination was probably the result of catalysis by at least two organohalide-respiring genera either in parallel or by stepwise catalysis. A more detailed study of one enrichment on cis-DCE suggested that in this culture Desulfitobacterium spp. as well as Dehalococcoides spp. dechlorinated cis-DCE whereas dechlorination of VC was only catalyzed by the latter.

Keywords

Organohalide respiration Dehalorespiration Halorespiration Reductive dechlorination Bioremediation 

Supplementary material

253_2010_3042_MOESM1_ESM.pdf (139 kb)
ESM 1(PDF 138 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Katia Rouzeau-Szynalski
    • 1
    • 2
  • Julien Maillard
    • 1
  • Christof Holliger
    • 1
  1. 1.Ecole Polytechnique Fédérale de Lausanne (EPFL), ENAC Laboratory for Environmental BiotechnologyLausanneSwitzerland
  2. 2.Product Technology Center-NestléKonolfingenSwitzerland

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