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Applied Microbiology and Biotechnology

, Volume 89, Issue 6, pp 1741–1750 | Cite as

Continuous d-lactic acid production by a novelthermotolerant Lactobacillus delbrueckii subsp. lactis QU 41

  • Yukihiro Tashiro
  • Wataru Kaneko
  • Yanqi Sun
  • Keisuke Shibata
  • Kentaro Inokuma
  • Takeshi Zendo
  • Kenji Sonomoto
Biotechnological Products and Process Engineering

Abstract

We isolated and characterized a d-lactic acid-producing lactic acid bacterium (d-LAB), identified as Lactobacillus delbrueckii subsp. lactis QU 41. When compared to Lactobacillus coryniformis subsp. torquens JCM 1166 T and L. delbrueckii subsp. lactis JCM 1248 T, which are also known as d-LAB, the QU 41 strain exhibited a high thermotolerance and produced d-lactic acid at temperatures of 50 °C and higher. In order to optimize the culture conditions of the QU 41 strain, we examined the effects of pH control, temperature, neutralizing reagent, and initial glucose concentration on d-lactic acid production in batch cultures. It was found that the optimal production of 20.1 g/l d-lactic acid was acquired with high optical purity (>99.9% of d-lactic acid) in a pH 6.0-controlled batch culture, by adding ammonium hydroxide as a neutralizing reagent, at 43 °C in MRS medium containing 20 g/l glucose. As a result of product inhibition and low cell density, continuous cultures were investigated using a microfiltration membrane module to recycle flow-through cells in order to improve d-lactic acid productivity. At a dilution rate of 0.87 h−1, the high cell density continuous culture exhibited the highest d-lactic acid productivity of 18.0 g/l/h with a high yield (ca. 1.0 g/g consumed glucose) and a low residual glucose (<0.1 g/l) in comparison with systems published to date.

Keywords

d-lactic acid fermentation Lactobacillus delbrueckii subsp. lactis QU 41 Continuous culture High cell density Thermotolerant 

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Yukihiro Tashiro
    • 1
  • Wataru Kaneko
    • 2
  • Yanqi Sun
    • 2
  • Keisuke Shibata
    • 2
  • Kentaro Inokuma
    • 2
  • Takeshi Zendo
    • 2
  • Kenji Sonomoto
    • 2
    • 3
  1. 1.Department of Life StudySeinan Jo Gakuin University Junior CollegeFukuokaJapan
  2. 2.Laboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate SchoolKyushu UniversityFukuokaJapan
  3. 3.Laboratory of Functional Food Design, Department of Functional Metabolic Design, Bio-Architecture CenterKyushu UniversityFukuokaJapan

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