Production of rhamnolipids in solid-state cultivation using a mixture of sugarcane bagasse and corn bran supplemented with glycerol and soybean oil
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Rhamnolipid biosurfactants are attracting attention due to their low toxicity, high biodegradability, and good ecological acceptability. However, production in submerged culture is made difficult by severe foaming problems. Solid-state cultivation (SSC) is a promising alternative production method. In the current work, we report the optimization of rhamnolipid production by Pseudomonas aeruginosa UFPEDA 614 on a solid substrate containing sugarcane bagasse and corn bran. The best rhamnolipid production, 45 g/l of impregnating solution used, was obtained with a 50:50 (m/m) mixture of sugarcane bagasse and corn bran supplemented with an impregnating solution containing 6% (v/v) of each of glycerol and soybean oil. This level is comparable with those of previous studies undertaken in solid-state cultivation; the composition of the biosurfactant is similar, but our medium is cheaper. Our work therefore provides a suitable basis for future studies of the development of an SSC-based process for rhamnolipid production.
KeywordsRhamnolipids Biosurfactants Solid-state cultivation Solid-state fermentation Pseudomonas aeruginosa
This work was supported financially by Corn Products Brasil and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil), a Brazilian government agency for the advancement of science and technology. Research scholarships were granted to David Mitchell, Nadia Krieger, Arquimedes Paixão de Santana-Filho, Guilherme L. Sassaki, and Lauro M. de Souza by CNPq, to Doumit Camilios-Neto by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil), and to Cryshelen Bugay and Talita Joslin by Corn Products Brasil.
- Desai JD, Banat IM (1997) Microbial production of surfactants and their commercial potential. Microbiol Mol Biol Rev 61:47–64Google Scholar
- Itoh S, Honda H, Tomota F, Suzuki T (1971) Rhamnolipids produced by Pseudomonas aeruginosa grown on n-paraffin. J Antibiot 24:855–859Google Scholar
- Mulligan CN, Gibbs BF (1993) Factors influencing the economics of biosurfactants. In: Kosaric N (ed) Biosurfactants. Marcel Dekker, New York, pp 329–371Google Scholar
- Souza LM, Cipriani TR, Serrato RV, Costa DE, Iacomini M, Gorin PAJ, Sassaki GL (2008) Analysis of flavonol glycoside isomers from leaves of Maytenus ilicifolia by offline and online high performance liquid chromatography–electrospray mass spectrometry. J Chromatogr A 1207:101–109CrossRefGoogle Scholar