Applied Microbiology and Biotechnology

, Volume 89, Issue 3, pp 739–746 | Cite as

Genetic analysis around aminoalcohol dehydrogenase gene of Rhodococcus erythropolis MAK154: a putative GntR transcription factor in transcriptional regulation

  • Nobuyuki Urano
  • Michihiko Kataoka
  • Takeru Ishige
  • Shinji Kita
  • Keiji Sakamoto
  • Sakayu Shimizu
Applied Genetics and Molecular Biotechnology


NADP+-dependent aminoalcohol dehydrogenase (AADH) of Rhodococcus erythropolis MAK154 catalyzes the reduction of (S)-1-phenyl-1-keto-2-methylaminopropane ((S)-MAK) to d-pseudoephedrine, which is used as a pharmaceutical. AADH is suggested to participate in aminoalcohol or aminoketone metabolism in this organism because it is induced by the addition of several aminoalcohols, such as 1-amino-2-propanol. Genetic analysis of around the aadh gene showed that some open reading frames (ORFs) are involved in this metabolic pathway. Four of these ORFs might form a carboxysome-like polyhedral organelle, and others are predicted to encode aminotransferase, aldehyde dehydrogenase, phosphotransferase, and regulator protein. OrfE, a homologous ORF of the FadR subfamily of GntR transcriptional regulators, lies downstream from aadh. To investigate whether or not orfE plays a role in the regulation of aadh expression, the gene disruption mutant of R. erythropolis MAK154 was constructed. The ΔorfE strain showed higher AADH activity than wild-type strain. In addition, a transformed strain, which harbored multi-orfE, showed no AADH activity even in the induced condition with 1-amino-2-propanol. These results suggest that OrfE is a negative regulator that represses aadh expression in the absence of 1-amino-2-propanol.


Rhodococcus erythropolis Aminoalcohol dehydrogenase Aminoalcohol metabolism GntR family transcriptional regulator 



This work was supported in part by a Grant-in-Aid for Scientific Research, No. 20380051 (to MK), from the Japan Society for the Promotion of Science (JSPS), and by the Targeted Proteins Research Program (TPRP) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Nobuyuki Urano
    • 1
  • Michihiko Kataoka
    • 1
    • 3
  • Takeru Ishige
    • 1
  • Shinji Kita
    • 2
  • Keiji Sakamoto
    • 2
  • Sakayu Shimizu
    • 1
  1. 1.Division of Applied Life Sciences, Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.Research InstituteDaiichi Fine Chemical Co.TakaokaJapan
  3. 3.Division of Applied Life Sciences, Graduate School of Life and Environmental SciencesOsaka Prefecture UniversitySakaiJapan

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