Applied Microbiology and Biotechnology

, Volume 88, Issue 6, pp 1413–1422 | Cite as

Response of microbial community structure to microbial plugging in a mesothermic petroleum reservoir in China

  • Fan ZhangEmail author
  • Yue Hui SheEmail author
  • Sha Sha Ma
  • Ji Ming Hu
  • Ibrahim M. Banat
  • Du Jie HouEmail author
Environmental Biotechnology


Microbial plugging, a microbial enhancement of oil recovery (MEOR) technique, has been applied in a candidate oil reservoir of Daqing Oil Field (China). The goal of this study is to monitor the survival of injected bacteria and reveal the response of microbial communities in field trial of microbial plugging through injection of selected microbial culture broth and nutrients. Culture-dependent enrichment and culture-independent 16S rDNA clone library methods were used. The results show that it was easy to activate targeted biopolymer-producing bacteria in a laboratory environment, and it was difficult for injected exogenous bacteria to survive. In addition, microbial communities in the oil reservoir also changed before and after the field trial. However, microbial communities, activated by fermentative medium for biopolymer-producing bacteria, appeared to show greater differences in the laboratory than in the natural reservoir. It was concluded that microbial populations monitoring was important to MEOR; results of response of microbial communities could provide a guide for the future field trials.


Microbial plugging Microbial enhancement of oil recovery (MEOR) Microbial communities Biopolymer-producing bacteria 16S rDNA clone library 



This study was sponsored by the 863 Program (No. 2008AA06Z204) of the Ministry of Science and Technology, the National Natural Science Foundation (No.50974022) in P.R.China, and the program (No. DQYT-0503003-2006-JS-10257) of Daqing Oilfield Limited Liability Company of China National Petroleum Corporation. The authors wish to thank Engineers in the Daqing Oil Field, for kindly collecting samples and providing geo-materials of the studied block.


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, Ministry of Education, School of Energy Resources, China University of Geosciences (Beijing)BeijingChina
  2. 2.College of Chemistry and Molecular SciencesWuhan UniversityWuhanChina
  3. 3.College of Chemistry and Environmental EngineeringYangtze UniversityJingzhouChina
  4. 4.School of Biomedical SciencesUniversity of UlsterColeraineUK

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