Applied Microbiology and Biotechnology

, Volume 88, Issue 5, pp 1087–1093

Scale-up of Baeyer–Villiger monooxygenase-catalyzed synthesis of enantiopure compounds

  • Kristian Geitner
  • Jessica Rehdorf
  • Radka Snajdrova
  • Uwe T. Bornscheuer
Biotechnological Products and Process Engineering

DOI: 10.1007/s00253-010-2724-y

Cite this article as:
Geitner, K., Rehdorf, J., Snajdrova, R. et al. Appl Microbiol Biotechnol (2010) 88: 1087. doi:10.1007/s00253-010-2724-y

Abstract

Several Baeyer–Villiger monooxygenases converting a wide spectrum of substrates have been discovered, cloned, and characterized throughout the last few years. Still, only a few of them are applicable for large-scale conversion predominantly due to their sensitivity towards high substrate and/or product concentrations. The recently cloned and characterized 4-hydroxyacetophenone monooxygenase from Pseudomonas putida JD1 shows excellent enantioselectivity towards 3-phenyl-2-butanone with E > 100 but is inhibited by concentrations >10 mM of both substrate and product. This obstacle could be circumvented by in situ substrate feed and product removal using a hydrophobic Lewatit® adsorbent resin. Thus, the concentration of 3-phenyl-2-butanone could be increased from 1.4 to >26 mM without significant reduction in conversion.

Keywords

Baeyer–Villiger monooxygenase Reaction scale-up SFPR Aryl ketones Adsorbent resin 

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Kristian Geitner
    • 1
  • Jessica Rehdorf
    • 1
  • Radka Snajdrova
    • 1
  • Uwe T. Bornscheuer
    • 1
  1. 1.Department of Biotechnology and Enzyme Catalysis, Institute for BiochemistryGreifswald UniversityGreifswaldGermany

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