Applied Microbiology and Biotechnology

, Volume 88, Issue 1, pp 87–94 | Cite as

Ethanol production from biomass by repetitive solid-state fed-batch fermentation with continuous recovery of ethanol

  • Churairat Moukamnerd
  • Masahiro Kino-oka
  • Minetaka Sugiyama
  • Yoshinobu Kaneko
  • Chuenchit Boonchird
  • Satoshi Harashima
  • Hideo Noda
  • Kazuaki Ninomiya
  • Suteaki Shioya
  • Yoshio Katakura
Biotechnological Products and Process Engineering


To save cost and input energy for bioethanol production, a consolidated continuous solid-state fermentation system composed of a rotating drum reactor, a humidifier, and a condenser was developed. Biomass, saccharifying enzymes, yeast, and a minimum amount of water are introduced into the system. Ethanol produced by simultaneous saccharification and fermentation is continuously recovered as vapor from the headspace of the reactor, while the humidifier compensates for the water loss. From raw corn starch as a biomass model, 95 ± 3, 226 ± 9, 458 ± 26, and 509 ± 64 g l−1 of ethanol solutions were recovered continuously when the ethanol content in reactor was controlled at 10–20, 30–50, 50–70 and 75–85 g kg-mixture−1, respectively. The residue showed a lesser volume and higher solid content than that obtained by conventional liquid fermentation. The cost and energy for intensive waste water treatment are decreased, and the continuous fermentation enabled the sustainability of enzyme activity and yeast in the system.


Bioethanol Solid-state fermentation Simultaneous saccharification and fermentation Continuous recovery of ethanol 



flow rate, (l min−1)


initial flow rate, (l min−1)


batch number or number of substrate addition


ethanol production rate obtained by calculation, (g h−1)


ethanol production rate obtained in experiment, (g h−1)


initial weight of fermentation mixture, (g-mixture)


ethanol concentration in recovery system, (g l−1)


volume of recovered ethanol, (ml)


sampling time, (h)


substrate amount, (g)


initial substrate amount, (g)

Greek letters


ethanol content in fermentation mixture, (g kg-mixture−1)


maximum ethanol content in fermentation mixture, (g kg-mixture−1)


minimum ethanol content in fermentation mixture, (g kg-mixture−1)



This work was partially supported by the New Energy and Industrial Technology Development Organization (NEDO). We thank Mr. Junpei Kishimoto, Tetsutaro Ohmichi, and Keiichi Hirao for their technical assistance. This work will be registered for the dissertation of one of the authors (C.M.).


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Churairat Moukamnerd
    • 1
  • Masahiro Kino-oka
    • 1
  • Minetaka Sugiyama
    • 1
  • Yoshinobu Kaneko
    • 1
  • Chuenchit Boonchird
    • 2
  • Satoshi Harashima
    • 1
  • Hideo Noda
    • 3
  • Kazuaki Ninomiya
    • 4
  • Suteaki Shioya
    • 5
  • Yoshio Katakura
    • 1
  1. 1.Department of Biotechnology, Graduate School of EngineeringOsaka UniversityOsakaJapan
  2. 2.Faculty of Science, Department of BiotechnologyMahidol UniversityBangkokThailand
  3. 3.Kansai Chemical Engineering Co., Ltd.AmagasakiJapan
  4. 4.Institute of International Environment TechnologyKanazawa UniversityKanazawaJapan
  5. 5.Department of Applied Life ScienceSojo UniversityKumamotoJapan

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