Applied Microbiology and Biotechnology

, Volume 86, Issue 5, pp 1535–1541 | Cite as

Poly-β-hydroxybutyrate (PHB) increases growth performance and intestinal bacterial range-weighted richness in juvenile European sea bass, Dicentrarchus labrax

  • Peter De Schryver
  • Amit Kumar Sinha
  • Prabesh Singh Kunwar
  • Kartik Baruah
  • Willy VerstraeteEmail author
  • Nico Boon
  • Gudrun De Boeck
  • Peter Bossier
Applied Microbial and Cell Physiology


The bacterial storage polymer poly-β-hydroxybutyrate (PHB) has the potential to be used as an alternative anti-infective strategy for aquaculture rearing. In this research, the effects of (partially) replacing the feed of European sea bass juveniles with PHB were investigated. During a 6-week trial period, the PHB showed the ability to act as an energy source for the fish. This indicated that PHB was degraded and used during gastrointestinal passage. The gut pH decreased from 7.7 to 7.2 suggesting that the presence of PHB in the gut led to the increased production of (short-chain fatty) acids. The diets supplemented with 2% and 5% PHB (w/w) induced a gain of the initial fish weight with a factor 2.4 and 2.7, respectively, relative to a factor 2.2 in the normal feed treatment. Simultaneously, these treatments showed the highest bacterial range-weighted richness in the fish intestine. Based on molecular analysis, higher dietary PHB levels induced larger changes in the bacterial community composition. From our results, it seems that PHB can have a beneficial effect on fish growth performance and that the intestinal bacterial community structure may be closely related to this phenomenon.


Prebiotics Antibiotics Infection Growth promoting agent Host–microbe interactions 



This work was performed and funded within the frame of the Research Foundation of Flanders (FWO) project ‘‘Probiont-induced functional responses in aquatic organisms” and the European FP7 project “Promicrobe-Microbes as positive actors for more sustainable aquaculture” (Project Reference: 227197). The authors would also like to thank Dr. ir. Tom Defoirdt, lic. Kristof Dierckens and ir. Charlotte Grootaert for the critical reading of the manuscript and the helpful suggestions.


  1. Acar J, Casewell M, Freeman J, Friis C, Goossens H (2000) Avoparcin and virginiamycin as animal growth promoters: a plea for science in decision-making. Clin Microbial Infect 6:477–482CrossRefGoogle Scholar
  2. Azain MJ (2004) Role of fatty acids in adipocyte growth and development. J Anim Sci 82:916–924Google Scholar
  3. Bongers A, van den Heuvel E (2003) Prebiotics and the bioavailability of minerals and trace elements. Food Rev Int 19:397–422CrossRefGoogle Scholar
  4. Boon N, De Windt W, Verstraete W, Top EM (2002) Evaluation of nested PCR-DGGE (denaturing gradient gel electrophoresis) with group-specific 16S rRNA primers for the analysis of bacterial communities from different wastewater treatment plants. FEMS Microbiol Ecol 39:101–112Google Scholar
  5. Burr G, Gatlin D, Ricke S (2005) Microbial ecology of the gastrointestinal tract of fish and the potential application of prebiotics and probiotics in finfish aquaculture. J World Aquacult Soc 36:425–436CrossRefGoogle Scholar
  6. Defoirdt T, Halet D, Sorgeloos P, Bossier P, Verstraete W (2006) Short-chain fatty acids protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii. Aquaculture 261:804–808CrossRefGoogle Scholar
  7. Defoirdt T, Boon N, Sorgeloos P, Verstraete W, Bossier P (2007a) Alternatives to antibiotics to control bacterial infections: luminescent vibriosis in aquaculture as an example. Trends Biotechnol 25:472–479CrossRefGoogle Scholar
  8. Defoirdt T, Halet D, Vervaeren H, Boon N, Van de Wiele T, Sorgeloos P, Bossier P, Verstraete W (2007b) The bacterial storage compound poly-beta-hydroxybutyrate protects Artemia franciscana from pathogenic Vibrio campbellii. Environ Microbiol 9:445–452CrossRefGoogle Scholar
  9. Dierckens K, Rekecki A, Laureau S, Sorgeloos P, Boon N, Van den Broeck W, Bossier P (2009) Development of a bacterial challenge test for gnotobiotic sea bass (Dicentrarchus labrax) larvae. Environ Microbiol 11:526–533CrossRefGoogle Scholar
  10. Gebauer B, Jendrossek D (2006) Assay of poly(3-hydroxybutyrate) depolymerase activity and product determination. Appl Environ Microbiol 72:6094–6100CrossRefGoogle Scholar
  11. Gibson GR, Roberfroid MB (1995) Dietary modulation of the human colonic microbiota—introducing the concept of prebiotics. J Nutr 125:1401–1412Google Scholar
  12. Goncalves LMD, Ramos A, Almeida JS, Xavier A, Carrondo MJT (1997) Elucidation of the mechanism of lactic acid growth inhibition and production in batch cultures of Lactobacillus rhamnosus. Appl Microbiol Biotechnol 48:346–350CrossRefGoogle Scholar
  13. Halet D, Defoirdt T, Van Damme P, Vervaeren H, Forrez I, Van de Wiele T, Boon N, Sorgeloos P, Bossier P, Verstraete W (2007) Poly-beta-hydroxybutyrate-accumulating bacteria protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii. FEMS Microbiol Ecol 60:363–369CrossRefGoogle Scholar
  14. Hismiogullari SE, Hismiogullari AA, Sahin F, Oner ET, Yenice S, Karasartova D (2008) Investigation of antibacterial and cytotoxic effects of organic acids including ascorbic acid, lactic acid and acetic acids on mammalian cells. J Anim Vet Adv 7:681–684Google Scholar
  15. Jendrossek D, Handrick R (2002) Microbial degradation of polyhydroxyalkanoates. Annu Rev Microbiol 56:403–432CrossRefGoogle Scholar
  16. Karunasagar I, Shivu MM, Girisha SK, Krohne G, Karunasagar I (2007) Biocontrol of pathogens in shrimp hatcheries using bacteriophages. Aquaculture 268:288–292CrossRefGoogle Scholar
  17. Li P, Gatlin DM (2004) Dietary brewers yeast and the prebiotic Grobiotic (TM) AE influence growth performance, immune responses and resistance of hybrid striped bass (Morone chrysops x M-saxatilis) to Streptococcus iniae infection. Aquaculture 231:445–456CrossRefGoogle Scholar
  18. Li P, Burr GS, Gatlin DM, Hume ME, Patnaik S, Castille FL, Lawrence AL (2007) Dietary supplementation of short-chain fructooligosaccharides influences gastrointestinal microbiota composition and immunity characteristics of pacific white shrimp, Litopenaeus vannamei, cultured in a recirculating system. J Nutr 137:2763–2768Google Scholar
  19. Listewnik HF, Wendlandt KD, Jechorek M, Mirschel G (2007) Process design for the microbial synthesis of poly-beta-hydroxybutyrate (PHB) from natural gas. Eng Life Sci 7:278–282CrossRefGoogle Scholar
  20. Madison LL, Huisman GW (1999) Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol Mol Biol Rev 63:21–53Google Scholar
  21. Mahious AS, Gatesoupe FJ, Hervi M, Metailler R, Ollevier F (2006) Effect of dietary inulin and oligosaccharides as prebiotics for weaning turbot, Psetta maxima (Linnaeus, C. 1758). Aquac Int 14:219–229CrossRefGoogle Scholar
  22. Marzorati M, Wittebolle L, Boon N, Daffonchio D, Verstraete W (2008) How to get more out of molecular fingerprints: practical tools for microbial ecology. Environ Microbiol 10:1571–1581Google Scholar
  23. Nicolas JL, Gatesoupe FJ, Froueli S, Bachere E, Gueguen Y (2007) What alternatives to antibiotics are conceivable for aquaculture? Prod Anim 20:253–258Google Scholar
  24. Panigrahi A, Azad IS (2007) Microbial intervention for better fish health in aquaculture: the Indian scenario. Fish Physiol Biochem 33:429–440CrossRefGoogle Scholar
  25. Rawls JF, Samuel BS, Gordon JI (2004) Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota. Proc Natl Acad Sci U S A 101:4596–4601CrossRefGoogle Scholar
  26. Ricke SC (2003) Perspectives on the use of organic acids and short chain fatty acids as antimicrobials. Poult Sci 82:632–639Google Scholar
  27. Sammouth S, d'Orbcastel ER, Gasset E, LemariÈ G, Breuil G, Marino G, Coeurdacier J-L, Fivelstad S, Blancheton J-P (2009) The effect of density on sea bass (Dicentrarchus labrax) performance in a tank-based recirculating system. Aquac Eng 40:72–78CrossRefGoogle Scholar
  28. Sapkota A, Sapkota AR, Kucharski M, Burke J, McKenzie S, Walker P, Lawrence R (2008) Aquaculture practices and potential human health risks: current knowledge and future priorities. Environ Int 34:1215–1226CrossRefGoogle Scholar
  29. Scholz-Ahrens KE, Ade P, Marten B, Weber P, Timm W, Asil Y, Gluer CC, Schrezenmeir J (2007) Prebiotics, probiotics, and synbiotics affect mineral absorption, bone mineral content, and bone structure. J Nutr 137:838S–846SGoogle Scholar
  30. Thompson JL, Hinton M (1996) Effect of short-chain fatty acids on the size of enteric bacteria. Lett Appl Microbiol 22:408–412CrossRefGoogle Scholar
  31. Tokiwa Y, Calabia BP (2004) Degradation of microbial polyesters. Biotechnol Lett 26:1181–1189CrossRefGoogle Scholar
  32. Tokiwa Y, Calabia BP (2007) Biodegradability and biodegradation of polyesters. J Polym Environ 15:259–267CrossRefGoogle Scholar
  33. Vazquez JA, Gonzalez MP, Murado MA (2005) Effects of lactic acid bacteria cultures on pathogenic microbiota from fish. Aquaculture 245:149–161CrossRefGoogle Scholar
  34. Zhou ZG, Ding ZK, Huiyuan LV (2007) Effects of dietary short-chain fructooligosaccharides on intestinal microflora, survival, and growth performance of juvenile white shrimp, Litopenaeus vannamei. J World Aquacult Soc 38:296–301CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Peter De Schryver
    • 1
  • Amit Kumar Sinha
    • 2
    • 3
  • Prabesh Singh Kunwar
    • 2
  • Kartik Baruah
    • 3
  • Willy Verstraete
    • 1
    Email author
  • Nico Boon
    • 1
  • Gudrun De Boeck
    • 2
  • Peter Bossier
    • 3
  1. 1.Laboratory of Microbial Ecology and Technology (LabMET)Ghent UniversityGhentBelgium
  2. 2.Laboratory for Ecophysiology, Biochemistry and ToxicologyUniversity of AntwerpAntwerpBelgium
  3. 3.Laboratory of Aquaculture and Artemia Reference CenterGhent UniversityGhentBelgium

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