Applied Microbiology and Biotechnology

, Volume 85, Issue 6, pp 1735–1750 | Cite as

Biochemical features of microbial keratinases and their production and applications

  • Adriano Brandelli
  • Daniel J. Daroit
  • Alessandro Riffel


Keratinases are exciting proteolytic enzymes that display the capability to degrade the insoluble protein keratin. These enzymes are produced by diverse microorganisms belonging to the Eucarya, Bacteria, and Archea domains. Keratinases display a great diversity in their biochemical and biophysical properties. Most keratinases are optimally active at neutral to alkaline pH and 40–60°C, but examples of microbial keratinolysis at alkalophilic and thermophilic conditions have been well documented. Several keratinases have been associated to the subtilisin family of serine-type proteases by analysis of their protein sequences. Studies with specific substrates and inhibitors indicated that keratinases are often serine or metalloproteases with preference for hydrophobic and aromatic residues at the P1 position. Keratinolytic enzymes have several current and potential applications in agroindustrial, pharmaceutical, and biomedical fields. Their use in biomass conversion into biofuels may address the increasing concern on energy conservation and recycling.


Feather Keratin Microbial protease Serine protease Metalloprotease Proteolysis 



AB is research fellow of Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Adriano Brandelli
    • 1
    • 3
  • Daniel J. Daroit
    • 1
  • Alessandro Riffel
    • 2
  1. 1.Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de AlimentosUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  2. 2.Embrapa-Empresa Brasileira de Pesquisa AgropecuáriaCentro de Pesquisa Agropecuária dos Tabuleiros Costeiros-UEP AlagoasMaceióBrazil
  3. 3.ICTA-UFRGSPorto AlegreBrazil

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