Antibacterial and biofilm removal activity of a podoviridae Staphylococcus aureus bacteriophage SAP-2 and a derived recombinant cell-wall-degrading enzyme
- 987 Downloads
Antibacterial and biofilm removal activity of a new podoviridae Staphylococcus aureus bacteriophage (SAP-2), which belongs to the φ29-like phage genus of the Podoviridae family, and a cell-wall-degrading enzyme (SAL-2), which is derived from bacteriophage SAP-2, have been characterized. The cell-wall-degrading enzyme SAL-2 was expressed in Escherichia coli in a soluble form using a low-temperature culture. The cell-wall-degrading enzyme SAL-2 had specific lytic activity against S. aureus, including methicillin-resistant strains, and showed a minimum inhibitory concentration of about 1 μg/ml. In addition, this enzyme showed a broader spectrum of activity within the Staphylococcus genus compared with bacteriophage SAP-2 in its ability to remove the S. aureus biofilms. Thus, the cell-wall-degrading enzyme SAL-2 can be used to prevent and treat biofilm-associated S. aureus infections either on its own or in combination with other cell-wall-degrading enzymes with anti-S. aureus activity.
KeywordsCell-wall-degrading enzyme Podoviridae Bacteriophage Biofilm Staphylococcus aureus
This work was partially supported by a graduate fellowship from the Brain Korea 21 project of the Korean Ministry of Education, Science, and Technology and the Research Institute for Agriculture and Life Sciences, Seoul National University. Electron microscopy was carried out by the National Instrumental Center for Environmental Management (NICEM), Seoul National University.
- Ando E, Monden K, Mitsuhata R, Kariyama R, Kumon H (2004) Biofilm formation among methicillin-resistant Staphylococcus aureus isolates from patients with urinary tract infection. Acta Med Okayama 58:207–214Google Scholar
- Andrews JM (2001) Determination of minimum inhibitory concentrations. J Antimicrob Chemother 48(Suppl 1):5–16Google Scholar
- Arciola CR, Montanaro L, Baldassarri L, Borsetti E, Cavedagna D, Donati E (1999) Slime production by Staphylococci isolated from prosthesis-associated infections. New Microbiol 22:337–341Google Scholar
- Baird-Parker AC (1962) An improved diagnostic and selective medium for isolating coagulase-positive staphylococci. J Appl Bacteriol 25:12–19Google Scholar
- Barry AL, García F, Thrupp LD (1970) An improved single-disk method for testing the antibiotic susceptibility of rapidly-growing pathogens. Am J Clin Pathol 53:149–158Google Scholar
- Cramton SE, Gerke C, Schnell NF, Nichols WW, Götz F (1999) The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation. Infect Immun 67:5427–5433Google Scholar
- Johnson AP, Aucken HM, Cavendish S, Ganner M, Wale MC, Warner M, Livermore DM, Cookson BD, the UK EARSS participants (2001) Dominance of EMRSA-15 and -16 among MRSA causing nosocomial bacteraemia in the UK: analysis of isolates from the European Antimicrobial Resistance Surveillance System (EARSS). J Antimicrob Chemother 48:143–144CrossRefGoogle Scholar
- Le Loir Y, Baron F, Gautier M (2003) Staphylococcus aureus and food poisoning. Genet Mol Res 2:63–76Google Scholar
- Novick RP (1963) Analysis by transduction of mutations affecting penicillinase formation in Staphylococcus aureus. J Gen Microbiol 33:121–136Google Scholar
- O’Gara JP, Humphreys H (2001) Staphylococcus epidermidis biofilms: importance and implications. J Med Microbiol 50:581–587Google Scholar