Applied Microbiology and Biotechnology

, Volume 86, Issue 2, pp 731–741

Synthesis of γ-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463: functional grape must beverage and dermatological applications

  • Raffaella Di Cagno
  • Francesco Mazzacane
  • Carlo Giuseppe Rizzello
  • Maria De Angelis
  • Giammaria Giuliani
  • Marisa Meloni
  • Barbara De Servi
  • Marco Gobbetti
Applied Microbial and Cell Physiology


Agriculture surplus were used as substrates to synthesize γ-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463 for the manufacture of a functional beverage or as a novel application for dermatological purposes. Dilution of the grape must to 1 or 4% (w/v) of total carbohydrates favored higher cell yield and synthesis of GABA with respect to whey milk. Optimal conditions for synthesizing GABA in grape must were: initial pH 6.0, initial cell density of Log 7.0 cfu/mL, and addition of 18.4 mM l-glutamate. L. plantarum DSM19463 synthesized 4.83 mM of GABA during fermentation at 30°C for 72 h. The fermented grape must also contain various levels of niacin, free minerals, and polyphenols, and Log 10.0 cfu/g of viable cells of L. plantarum DSM19463. Freeze dried preparation of grape must was applied to the SkinEthic® Reconstructed Human Epidermis or multi-layer human skin model (FT-skin tissue). The effect on transcriptional regulation of human beta-defensin-2 (HBD-2), hyaluronan synthase (HAS1), filaggrin (FGR), and involucrin genes was assayed through RT-PCR. Compared to GABA used as pure chemical compound, the up-regulation HBD-2 was similar while the effect on the expression of HAS1 and FGR genes was higher.


γ-Aminobutyric acid Functional grape must Human skin protection Lactobacillus plantarum 


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Raffaella Di Cagno
    • 1
  • Francesco Mazzacane
    • 1
  • Carlo Giuseppe Rizzello
    • 1
  • Maria De Angelis
    • 1
  • Giammaria Giuliani
    • 2
  • Marisa Meloni
    • 3
  • Barbara De Servi
    • 3
  • Marco Gobbetti
    • 1
  1. 1.Dipartimento di Protezione delle Piante e Microbiologia Applicata, Facoltà di AgrariaUniversità degli Studi di BariBariItaly
  2. 2.Giuliani S.p.A.MilanoItaly
  3. 3.In Vitro Research LaboratoriesVitroScreen SrlMilanoItaly

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