Applied Microbiology and Biotechnology

, Volume 85, Issue 4, pp 909–919 | Cite as

New perspective for phage display as an efficient and versatile technology of functional proteomics

Mini-Review
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Abstract

Phage display with antibody libraries has been widely used with versatile applications. However, phage display with cDNA libraries is rare and inefficient. Because of uncontrollable reading frames and stop codons in cDNA repertoires, high percentage of phage clones identified from conventional cDNA libraries are non-open reading frames (non-ORFs) encoding unnatural short peptides with minimal implications in protein networks. Consequently, phage display has not been used as a technology of functional proteomics to elucidate protein–protein interactions like yeast two-hybrid system and mass spectrometry-based technologies. Several strategies, including C-terminal display and ORF cDNA libraries, have been explored to circumvent the technical problem. The accumulative endeavors eventually led to the efficient elucidation of a large number of tubby- and phosphatidylserine-binding proteins in recent studies by ORF phage display with minimal reading frame issue. ORF phage display inherits all the versatile applications of antibody phage display, but enables efficient identification of real endogenous proteins with efficiency, sensitivity, and accuracy comparable to other technologies of functional proteomics. Its ELISA-like procedure can be conveniently adapted by individual laboratories or fully automated for high-throughput screening. Thus, ORF phage display is an efficient, sensitive, versatile, and convenient technology of functional proteomics for elucidation of global and pathway-specific protein–protein interactions, disease mechanisms, or therapeutic targets.

Keywords

Phage display Functional proteomics Yeast two-hybrid system Mass spectrometry ORF phage display Protein–protein interaction 
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Notes

Acknowledgments

This work is supported in part by NIH grant R01EY016211. N. Caberoy is a recipient of a Fight for Sight postdoctoral fellowship. We thank Dr. Sanjoy Bhattacharya for discussion.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Bascom Palmer Eye Institute, Department of OphthalmologyUniversity of Miami School of MedicineMiamiUSA

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