Advertisement

Microbial fuel cell with an azo-dye-feeding cathode

  • Liang Liu
  • Fang-bai Li
  • Chun-hua Feng
  • Xiang-zhong Li
Environmental Biotechnology

Abstract

Microbial fuel cells (MFCs) were constructed using azo dyes as the cathode oxidants to accept the electrons produced from the respiration of Klebsiella pneumoniae strain L17 in the anode. Experimental results showed that a methyl orange (MO)-feeding MFC produced a comparable performance against that of an air-based one at pH 3.0 and that azo dyes including MO, Orange I, and Orange II could be successfully degraded in such cathodes. The reaction rate constant (k) of azo dye reduction was positively correlated with the power output which was highly dependent on the catholyte pH and the dye molecular structure. When pH was varied from 3.0 to 9.0, the k value in relation to MO degradation decreased from 0.298 to 0.016 μmol min−1, and the maximum power density decreased from 34.77 to 1.51 mW m−2. The performances of the MFC fed with different azo dyes can be ranked from good to poor as MO > Orange I > Orange II. Furthermore, the cyclic voltammograms of azo dyes disclosed that the pH and the dye structure determined their redox potentials. A higher redox potential corresponded to a higher reaction rate.

Keywords

Microbial fuel cell Azo dyes K. pneumoniae Orange II Degradation 

Notes

Acknowledgements

The authors appreciate the financial supports by the National Science Foundation of China (no. 40771105). We are grateful to the anonymous reviewers for their constructive and helpful comments.

References

  1. Clauwaert P, Van Der Ha D, Boon N, Verbeken K, Verhaege M, Rabaey K, Verstraete W (2007a) An open air biocathode enables effective electricity generation with microbial fuel cells. Environ Sci Technol 41:7564–7569CrossRefGoogle Scholar
  2. Clauwaert P, Rabaey K, Aelterman P, De Schamphelaire L, Pham TH, Boeckx P, Boon N, Verstraete W (2007b) Biological denitrification in microbial fuel cells. Environ Sci Technol 41:3354–3360CrossRefGoogle Scholar
  3. Eriksson A, Nyholm L (2001) Coulometric and spectroscopic investigations of the oxidation and reduction of some azosalicylic acids at glassy carbon electrodes. Electrochim Acta 46:1113–1129CrossRefGoogle Scholar
  4. Fan J, Guo YH, Wang JJ, Fan MH (2009) Rapid decolorization of azo dye methyl orange in aqueous solution by nanoscale zerovalent iron particles. J Hazard Mater 166:904–910CrossRefGoogle Scholar
  5. Feng YJ, Wang X, Logan BE, Lee H (2008) Brewery wastewater treatment using air-cathode microbial fuel cells. Appl Microbiol Biotechnol 78:873–880CrossRefGoogle Scholar
  6. Ghoneim MM, El-Desoky HS, Amer SA, Rizk HF, Habazy AD (2008) Electroreduction and spectrophotometric studies of some pyrazolyl-azo dyes derived from 3-acetylamino-phenyl-5-pyrazolone in buffered solutions. Dyes Pigm 77:493–501CrossRefGoogle Scholar
  7. Goyal RN, Minocha A (1985) Electrochemical behaviour of the bisazo dye, direct red-81. J Electroanal Chem 193:231–240CrossRefGoogle Scholar
  8. Guaratini CCI, Fogg AG, Zanoni MVB (2001) Studies of the voltammetric behavior and determination of diazo reactive dyes at mercury electrode. Electroanalysis 13:1535–1543CrossRefGoogle Scholar
  9. Gupta VK, Jain R, Varshney S (2007) Electrochemical removal of the hazardous dye reactofix red 3 BFN from industrial effluents. J Colloid Interf Sci 312:292–296CrossRefGoogle Scholar
  10. He Z, Angenent LT (2006) Application of bacterial biocathodes in microbial fuel cells. Electroanalysis 18:2009–2015CrossRefGoogle Scholar
  11. He Z, Minteer SD, Angenent LT (2005) Electricity generation from artificial wastewater using an upflow microbial fuel cell. Environ Sci Technol 39:5262–5267CrossRefGoogle Scholar
  12. Hou MF, Li FB, Liu XM, Wang XG, Wan HF (2007) The effect of substituent groups on the reductive degradation of azo dyes by zerovalent iron. J Hazard Mater 145:305–314CrossRefGoogle Scholar
  13. Jia YH, Tran HT, Kim DH, Oh SJ, Park DH, Zhang RH, Ahn DH (2008) Simultaneous organics removal and bio-electrochemical denitrification in microbial fuel cells. Bioproc Biosyst Eng 31:315–321CrossRefGoogle Scholar
  14. Li XM, Zhou SG, Li FB, Wu CY, Zhuang L, Xu W, Liu L (2009) Fe(III) oxide reduction and carbon tetrachloride dechlorination by a newly isolated Klebsiella pneumoniae strain L17. J Appl Microbiol 106:130–139CrossRefGoogle Scholar
  15. Liu H, Ramnarayanan R, Logan BE (2004) Production of electricity during wastewater treatment using a single chamber microbial fuel cell. Environ Sci Technol 38:2281–2285CrossRefGoogle Scholar
  16. Lovley DR, Phillips EJP (1998) Novel mode of microbial energy metabolism: organic carbon oxidation coupled to dissimilatory reduction of iron or manganese. Appl Environ Microbiol 54:1472–1480Google Scholar
  17. Lu J, Zheng Y, He D (2006) Selective absorption of H2S from gas mixtures into aqueous solutions of blended amines of methyldiethanolamine and 2-tertiarybutylamino-2-ethoxyethanol in a packed column. Sep Purif Technol 52:209–217CrossRefGoogle Scholar
  18. Mandic Z, Nigovic B, Simunic B (2004) The mechanism and kinetics of the electrochemical cleavage of azo bond of 2-hydroxy-5-sulfophenyl-azo-benzoic acids. Electrochim Acta 49:607–615CrossRefGoogle Scholar
  19. Martinez-Huitle CA, Brillas E (2008) Decontamination of wastewaters containing synthetic organic dyes by electrochemical methods: a general review. Appl Catal B Environ 87:105–145. doi: 10.1016/j.apcatb.2008.09.017 CrossRefGoogle Scholar
  20. Menek N, Karaman Y (2005) Polarographic and voltammetric investigation of 8-hydroxy-7-(4-sulfo-1-naphthylazo)-5-quinoline sulfonic acid. Dyes Pigm 67:9–14CrossRefGoogle Scholar
  21. Min B, Logan BE (2004) Continuous electricity generation from domestic wastewater and organic substrates in a flat plate microbial fuel cell. Environ Sci Technol 38:5809–5814CrossRefGoogle Scholar
  22. Pinheiro HM, Touraud E, Thomas O (2004) Aromatic amines from azo dye reduction: status review with emphasis on direct UV spectrophotometric detection in textile industry wastewaters. Dyes Pigm 61:121–139CrossRefGoogle Scholar
  23. Potter MC (1912) Electrical effects accompanying the decomposition of organic compounds. Proc R Soc Lond B 84:260–276CrossRefGoogle Scholar
  24. Rabaey K, Read ST, Clauwaert P, Freguia S, Bond PL, Blackall LL, Keller J (2008) Cathodic oxygen reduction catalyzed by bacteria in microbial fuel cells. ISME J 2:519–527CrossRefGoogle Scholar
  25. Schröder U, Nieβen J, Scholz F (2003) A generation of microbial fuel cells with current outputs boosted by more than one order of magnitude. Angew Chem Int ed 42:2880–2883CrossRefGoogle Scholar
  26. Virdis B, Rabaey K, Yuan ZG, Keller J (2008) Microbial fuel cells for simultaneous carbon and nitrogen removal. Water Res 42:3013–3024CrossRefGoogle Scholar
  27. Wang G, Huang LP, Zhang YF (2008) Cathodic reduction of hexavalent chromium [Cr (VI)] coupled with electricity generation in microbial fuel cells. Biotechnol Lett 30:1959–1966CrossRefGoogle Scholar
  28. Xu MY, Guo J, Sun GP (2007) Biodegradation of textile azo dye by Shewanella decolorationis S12 under microaerophilic conditions. Appl Microbiol Biotechnol 76:719–726CrossRefGoogle Scholar
  29. You SJ, Zhao QL, Zhang JN, Jiang JQ, Zhao SQ (2006) A microbial fuel cell using permanganate as the cathodic electron acceptor. J Power Sources 162:1409–1415CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Guangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhouChina
  2. 2.Guangdong Key Laboratory of Agricultural Environment Pollution Integrated ControlGuangdong Institute of Eco-Environmental and Soil SciencesGuangzhouChina
  3. 3.School of Chemistry and Chemical EngineeringSouth China University of TechnologyGuangzhouChina
  4. 4.Department of Civil and Structural EngineeringThe Hong Kong Polytechnic UniversityHong KongChina
  5. 5.Graduate University of Chinese Academy of SciencesBeijingChina

Personalised recommendations