Gene cloning and expression of a new acidic family 7 endo-β-1,3-1,4-glucanase from the acidophilic fungus Bispora sp. MEY-1
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Most reported microbial β-1,3-1,4-glucanases belong to the glycoside hydrolase family 16. Here, we report a new acidic family 7 endo-β-1,3-1,4-glucanase (Bgl7A) from the acidophilic fungus Bispora sp. MEY-1. The cDNA of Bgl7A was isolated and over-expressed in Pichia pastoris, with a yield of about 1,000 U ml–1 in a 3.7-l fermentor. The purified recombinant Bgl7A had three activity peaks at pH 1.5, 3.5, and 5.0 (maximum), respectively, and a temperature optimum at 60°C. The enzyme was stable at pH 1.0–8.0 and highly resistant to both pepsin and trypsin. Belonging to the group of non-specific endoglucanase, Bgl7A can hydrolyze not only β-glucan and cellulose but also laminarin and oat spelt xylan. The specific activity of Bgl7A against barley β-glucan and lichenan (4,040 and 2,740 U mg–1) was higher than toward carboxymethyl cellulose sodium (395 U mg–1), which was different from other family 7 endo-β-glucanases.
KeywordsEndo-β-1,3-1,4-glucanase Acidophilic fungus Bispora sp. MEY-1 Pichia pastoris
This research was supported by the National High Technology Research and Development Program of China (863 program, grant no. 2007AA100601), Chinese Program on Research for Public Good (grant no. 2005DIB4J038), and 948 program of the Ministry of Agriculture (grant no. 2007-Z3).
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