Applied Microbiology and Biotechnology

, Volume 84, Issue 4, pp 677–683 | Cite as

Enhanced uridine diphosphate N-acetylglucosamine production using whole-cell catalysis

  • Hanjie Ying
  • Xiaochun Chen
  • Haiping Cao
  • Jian Xiong
  • Yuan Hong
  • Jianxin Bai
  • Zhenjiang Li
Biotechnological Products and Process Engineering
First Online:
Received:
Revised:
Accepted:

Abstract

Uridine diphosphate N-acetylglucosamine (UDPAG) can be produced by chemical, enzymatic, chemoenzymatic, and fermentative methods. In this study, we used whole-cell catalysis method to produce UDPAG for the first time by Saccharomyces cerevisiae. In order to increase the ATP utilization efficiency and UDPAG conversion yield, the response surface methodology was applied to optimize the whole-cell catalytic conditions for UDPAG production. Firstly, effects of uridine 5′-monophosphate (5′-UMP), glucosamine, vitamin B1, glycerol, magnesium chloride, potassium chloride, temperature, sodium dihydrogen phosphate, sodium acetate, fructose, and pH on UDPAG production were evaluated by a fractional factorial design. Results showed that UDPAG production was mainly affected by sodium dihydrogen phosphate, temperature, and vitamin B1. Then, the concentrations of sodium dihydrogen phosphate and vitamin B1 and temperature were further investigated with a central composite design and response surface analysis. The cultivation conditions to obtain the optimal UDPAG production were determined: sodium dihydrogen phosphate, 31.2 g/L; temperature, 29°C, and vitamin B1, 0.026 g/L. This optimization strategy led to an enhancement of UDPAG production from 2.51 to 4.25 g/L, yield from 44.6% to 75.6% based on the initial 5′-UMP concentration, and ATP utilization efficiency from 7.43% to 12.6%.

Keywords

ATP utilization efficiency Response surface methodology Saccharomyces cerevisiae Uridine diphosphate N-acetylglucosamine 
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Notes

Acknowledgments

This work was supported by the Major Basic R & D Program of China (2007CB707803), National High-Tech Research and Development Plan of china (2006AA02Z236), and Natural Science Foundation of Jiangsu Province (BK2007527).

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Hanjie Ying
    • 1
  • Xiaochun Chen
    • 1
  • Haiping Cao
    • 1
  • Jian Xiong
    • 1
  • Yuan Hong
    • 1
  • Jianxin Bai
    • 1
  • Zhenjiang Li
    • 1
  1. 1.State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical EngineeringNanjing University of TechnologyNanjingPeople’s Republic of China

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