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Increased PCP removal by Amylomyces rouxii transformants with heterologous Phanerochaete chrysosporium peroxidases supplementing their natural degradative pathway

Abstract

Fungal peroxidases and phenoloxidases are widely used in aromatic toxic compounds degradation. Peroxidases, such as lignin peroxidase and manganese peroxidase, as well as laccases are mainly produced by basidiomycetes and to a lower extent by other fungi, such as ascomycetes. Peroxidase-encoding genes have been described and homologous expression has been achieved in basidiomycetes. Heterologous expression has also been achieved in some non-producing peroxidase ascomycetes, like Penicillium and Aspergillus. In this work, heterologous expression of peroxidase-encoding genes, lignin peroxidase, and manganese peroxidase was achieved in a zygomycete producing only phenoloxidases (Amylomyces rouxii), aimed at coupling two different pathways used in nature for PCP removal in only one microbial strain. The ability of PCP removal was assayed with one of the obtained transformants, resulting in increased activity with respect to the ability of the parental strain cultured free of the inducer tyrosine (95% and 45%, respectively, of the initial PCP (12.5 mg L−1) in 120 h, or 100% and 49%, respectively, of the initial PCP after 144 h of liquid culture).

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Acknowledgments

This work was supported by National Council of Science and Technology, Mexico (CONACyT) grant 31572-B. Alba Mónica Montiel was supported by a fellowship from CONACyT, Mexico. We are also grateful to Dr. Daniel Cullen, University of Wisconsin (USA), for kindly donating the P. chrysosporium lignin and manganese peroxidases containing plasmids.

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Correspondence to F. J. Fernández.

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Montiel-González, A.M., Fernández, F.J., Keer, N. et al. Increased PCP removal by Amylomyces rouxii transformants with heterologous Phanerochaete chrysosporium peroxidases supplementing their natural degradative pathway. Appl Microbiol Biotechnol 84, 335–340 (2009). https://doi.org/10.1007/s00253-009-1981-0

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Keywords

  • PCP removal
  • Peroxidases
  • Tyrosinase
  • Zygomycetes