A number of rapid sampling devices for metabolic engineering applications have been developed over the last years with the purpose of the estimation of in vivo metabolic concentrations and dynamics. This review outlines the designs and characteristics as well as the developments and changes in diverse approaches over the years. Primary performance parameters for these constructions are sampling time and rate and, for an accurate representation of the in vivo condition in cells, the reproducibility of results and easy handling throughout the sampling operation.
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The authors gratefully acknowledge the financial support of the (German) Federal Ministry of Education and Research to this project.
Dedicated to Prof. Dr.-Ing. D. C. Hempel, Institute of Biochemical Engineering, Technische Universität Braunschweig, Germany, on the occasion of his 65th birthday.
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Schädel, F., Franco-Lara, E. Rapid sampling devices for metabolic engineering applications. Appl Microbiol Biotechnol 83, 199–208 (2009). https://doi.org/10.1007/s00253-009-1976-x
- Metabolic profiling
- Rapid sampling
- Sequential sampling
- Stimulus response experiments