The glyoxylate cycle is an anabolic pathway that is necessary for growth on nonfermentable carbon sources such as vegetable oils and is important for riboflavin production by the filamentous fungus Ashbya gossypii. The aim of this study was to identify malate synthase in the glyoxylate cycle of A. gossypii and to investigate its importance in riboflavin production from rapeseed oil. The ACR268C gene was identified as the malate synthase gene that encoded functional malate synthase in the glyoxylate cycle. The ACR268C gene knockout mutant lost malate synthase activity, and its riboflavin production and oil consumption were 10- and 2-fold lower, respectively, than the values of the wild-type strain. In contrast, the ACR268C gene-overexpressing strain showed a 1.6-fold increase in the malate synthase activity and 1.7-fold higher riboflavin production than the control strain. These results demonstrate that the malate synthase in the glyoxylate cycle has an important role not only in riboflavin production but also in oil consumption.
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This study was supported by the Comprehensive Support Programs for Creation of Regional Innovation in Japan Science and Technology Agency.
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Sugimoto, T., Kanamasa, S., Kato, T. et al. Importance of malate synthase in the glyoxylate cycle of Ashbya gossypii for the efficient production of riboflavin. Appl Microbiol Biotechnol 83, 529–539 (2009). https://doi.org/10.1007/s00253-009-1972-1
- Ashbya gossypii
- Malate synthase
- Gene disruptant
- Gene-targeting disruption
- Glyoxylate cycle