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Characterization and application of a native lactic acid bacterium isolated from tannery fleshings for fermentative bioconversion of tannery fleshings

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Abstract

Lactic acid bacteria (LAB) species isolated from limed and delimed tannery fleshings (TF) were evaluated for their fermentation efficiency and antibacterial property. The native LAB isolates efficiently fermented TF and resulted in a fermented mass with antioxidant properties, indicating their potential for effective eco-friendly bioconversion of TF. From among the LAB isolated, a proteolytic isolate showing better antimicrobial spectrum and reasonably good fermentation efficiency was identified as Enterococcus faecium HAB01 based on various biochemical and molecular tests. This isolate afforded a better degree of hydrolysis (81.36%) of TF than Pediococcus acidilactici (54.64%) that was previously reported by us. The bacteriocin produced by E. faecium was found to be antagonistic to several human pathogens including Listeria, Aeromonas, Staphylococcus and Salmonella. Further, E. faecium HAB01 bacteriocin was thermostable and had a molecular weight of around 5 kDa, apart from being stable at both acidic and alkaline conditions. The bacteriocin was unstable against proteases.

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Acknowledgement

This work is supported by funding from Council of Scientific and Industrial Research (CSIR) through the network programme (NWP-044) on Zero Emission Research Initiatives (ZERI). We thankfully acknowledge Mr. Masood Ahmed—Proprietor of M/s Mysore Super Reptiles Corporation, Bangalore (Karnataka, India)—for the gratis supply of limed TF. Authors thank Dr V Prakash, Director, CFTRI, for encouragement and permission to publish the work.

Conflict of interest statement

The authors declare that they have no conflict of interest.

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Correspondence to N. Bhaskar.

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Rai, A.K., Bhaskar, N., Halami, P.M. et al. Characterization and application of a native lactic acid bacterium isolated from tannery fleshings for fermentative bioconversion of tannery fleshings. Appl Microbiol Biotechnol 83, 757–766 (2009). https://doi.org/10.1007/s00253-009-1970-3

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Keywords

  • Tannery fleshings
  • Lactic acid bacteria
  • Antioxidant activity
  • Bacteriocin
  • Fermentation