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Applied Microbiology and Biotechnology

, Volume 83, Issue 3, pp 589–596 | Cite as

Use of cyclodextrin and its derivatives for increased transformation efficiency of competent bacterial cells

  • Finn Lillelund AachmannEmail author
  • Trond Erik Vee Aune
Methods

Abstract

Methodologies for introduction of DNA into cells are essential in molecular genetics and vital for applications such as genetic engineering and gene therapy. The use of cyclodextrins (CyDs) for increased efficiency of introducing DNA into eukaryotic cells (transfection) has been reported, but CyDs’ effect on the introduction of DNA into bacterial cells (transformation) is unknown. Here, we have investigated the potential of using CyDs in the transformation of chemically competent in-house, commercially available, and, on non-competent bacterial cells, with plasmid DNA of two different sizes. Possible interactions between CyDs and DNA were studied with nuclear magnetic resonance (NMR) spectroscopy. The presence of CyDs resulted in an up to fourfold increment of the transformation rate for in-house cells, with β-CyD and derivates giving the strongest effect. For commercial cells and transformation with megaplasmids, a more moderate effect around 1.4-fold was obtained. However, CyDs have little or no effect on DNA uptake by noncompetent cells. Results obtained from NMR spectroscopy show no interactions between CyDs and DNA-like molecules, which indicated that the CyDs’ effect is related to the bacterial cell wall.

Keywords

Cyclodextrins DNA Plasmid Bacteria Competent cell Transformation 

Notes

Acknowledgments

We thank Technology Transfer Office for financial support. Furthermore, we thank Svein Valla and Kim L. Larsen for proofreading the manuscript and for useful discussions and comments.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Finn Lillelund Aachmann
    • 1
    Email author
  • Trond Erik Vee Aune
    • 1
  1. 1.NOBIPOL, Department of BiotechnologyNorwegian University of Science and TechnologyTrondheimNorway

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